丛生蛋白在草酸钙溶液处理后犬肾小管上皮细胞中的表达  

The expression of clusterin in canine renal tubular epithelial cells treated with different concentra- tions of calcium oxalate

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作  者:刘俊江[1] 张蕴霞[2] 李守宾[1] 李瑾宜 于春红[4] 冯启红[4] 王刚[1] 魏东[1] 孙福振[1] 杨涛[1] 

机构地区:[1]河北省人民医院泌尿外科,石家庄050051 [2]河北省人民医院妇产科,石家庄050051 [3]西奈山医院泌尿外科,纽约91406 [4]开滦总医院眼科,唐山063000

出  处:《中华实验外科杂志》2016年第6期1488-1489,共2页Chinese Journal of Experimental Surgery

基  金:河北省科技厅指令性计划项目(14277774D)

摘  要:目的探讨丛生蛋白表达与肾结石形成的关系。方法用Westernblot方法及酶联免疫吸附试验(ELISA)方法检测不同浓度草酸钙溶液处理后的犬肾小管上皮细胞(MDCK)及上清液中丛生蛋白的表达水平。结果在对照组细胞及草酸钙溶液处理后的细胞中均可检测到丛生蛋白的表达,在处理过的细胞中表达水平明显升高;经浓度为300、500、900μg/ml草酸钙溶液处理后的细胞上清液中丛生蛋白的浓度分别为(241.33±59.28)、(247.67±22.48)、(270.67±43.50)pg/ml,明显高于对照组的(78.67±39.8)pg/ml(P〈0.01);而各处理组间丛生蛋白浓度差异无统计学意义(P〉0.05)。结论丛生蛋白在草酸钙溶液处理后的肾小管上皮细胞中表达升高,可能参与了肾结石的形成。Objective To investigate the relationship between clusterin expression and development of renal calculi. Methods Detect the expression level of clusterin in canine renal tubular epithelial cells (MDCK) and supernatant fluid after treated with different concentrations of calcium oxalate by Western blotting method and enzyme linked immunosorbent assay (ELISA) method. Results Clusterin expressed in both control cells and cells treated with calcium oxalate solution, but obviously stronger in treated cells. The clusterin concentration in supernatant fluid of cells treated with different concentration (300, 500, 900 μg/ml) was (241.33 ±59. 28), (247.67 ±22.48), (270. 67 ±43.50) pg/ml, respectively. Which was significant higher than that in control, (78.67 ± 39. 80) pg/ml ( P 〈 0.01 ) ; while among groups of different calcium oxalate concentrations treatment, there were no significant difference in clusterin concen- tration (P 〉 0. 05). Conclusion Clusterin expression increased in renal tubular epithelial cells treated with calcium oxalate, which indicate it may be involved in the formation of kidney stones.

关 键 词:肾结石 丛生蛋白 草酸钙 肾小管上皮细胞 

分 类 号:R692.4[医药卫生—泌尿科学]

 

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