消退素D1减少活化小胶质细胞对PC12细胞的毒性作用  

Resolvin D1 reduces the toxic effects of active BV -2 microglial cells to PC12 cells

作　　者：王艳萍[1] 郭慧玲[1] 赵小娜[1] 李新新[1] 李明明[1] 马民玉[1] 

机构地区：[1]郑州大学第一附属医院麻醉科河南省高等学校临床医学重点学科开放实验室,450052

出　　处：《中华实验外科杂志》2016年第6期1579-1582,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81200909）

摘　　要：目的观察消退素D1（RvD1）对活化BV-2小胶质细胞炎性反应的影响及其对PCI2神经元凋亡的影响。方法BV-2细胞分为对照组、脂多糖（LPS）组、RvD1±LPS组和RvD1组4组，分别采用含RvD1的培养基、100ng／mlLPS、100nmol／LRvD1＋100ng／mlLPS、100nmol／LRvD1处理24h后，收集细胞上清液培养PCI2细胞24h，4’，6-二脒基-2-苯基吲哚（DAPI）染色观察PCI2细胞凋亡，细胞计数试剂盒（CCK-8）法检测PCI2细胞存活率，流式细胞术测定PCI2细胞凋亡率；采用酶联免疫吸附试验（ELISA）检测各组BV-2细胞上清液中白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）的含量。结果与对照组比较，LPS组PCI2细胞表现出明显的凋亡形态学特征，细胞存活率（100．00％比84．93％）显著降低（P〈0．05），细胞凋亡率（5．12％比14．42％）显著升高（P〈0．05），上清液中IL-1β、IL-6、TNF—α的浓度[（45．68±4．80）pg／ml比（297．97±16．14）pg／ml、（26．97±4．44）pg／ml比（297．46±14．21）pg／ml、（80．12±9．34）pg／ml比（920．63±25．56）pg／m1]均显著升高（P〈0．05）；而RvD1±LPS组与鹏组比较，PCI2细胞较少表现出凋亡形态学特征，细胞存活率（91．60％比84．93％）显著升高（P〈0．05），细胞凋亡率（8．5l％比14．42％）显著降低（P〈0．05），上清液中IL-1β、IL-6、TNF-α的浓度[（114．47±9．65）pg／ml比（297．97±16．14）pg／ml、（111．10±9．57）pg／ml比（297．46±14．21）pg／ml、（320．66±18．21）pg／ml比（920．63±25．56）pg／ml）]均显著降低（P〈0．05）。结论RvD1可减少LPS诱导活化的BV-2细胞对PC12细胞的毒性作用，其机制可能与RvD1抑制活化BV-2细胞炎性介质表达有关。Objective To investigate the effects of Resolvin D1 （RvD1） on lipopolysaccharide （LPS） -induced BV -2 microglial cells activation and neuron -like PC12 cells apoptosis through sup- pressing activated BV - 2 microglial cell - mediated inflammatory reaction. Methods BV - 2 microglial cells were divided into four groups： control group, LPS group, RvD1 ± LPS group and RvDlgroup. BV -2 cells in each group were incubated with corresponding drugs for 24 h. The PC12 cells in each group were incubated with corresponding BV -2 ceils culture supernatant for 24 h. The apoptotic PCI2 ceils were ob- served by 4＇ ,6 - diamidino -2 - phenylindole （DAPI） staining. The survival rate of PC12 cells was tested by cell counting kit （CCK -8） assay. The apoptosis rate of PC12 cells was examined by Annexin V -fluo- resceine isothiocyanate （FITC）/propidium iodide （PI） apoptosis detection kit. The BV- 2 cells culture supematants were collected after incubating with corresponding drugs for 24 h, and the concentrations of in- terleukin （IL） -1β, IL-6 and tumor necrosis factor-α （TNF-α） were determined by enzyme linked immunosorbent assay （ELISA）. Results Compared to the control group, the PC12 cells presented evident apoptosis features and the cells survival rate （100.00% vs. 84.93% ） was significantly reduced （P 〈 0.05） and the apoptosis rate （5. 12% vs. 14.42%） was significantly increased （P〈0.05） in LPS group. The concentrations of IL - 1 β, IL - 6, and TNF - α [ （45.68 ± 4. 80） pg/ml vs. （ 297.97 ± 16.14） pg/ml, （26.97±4.44） pg/ml vs. （297.46 ± 14.21） pg/ml, （80.12 ±9.34） pg/ml vs. （920. 63 ± 25.56） pg/ml ] were significantly increased （ P 〈 0. 05 ） in LPS group. As compared with the LPS group, the apoptosis of PC12 ceils was significantly reduced, the cells survival rate （91.60% vs.84.93 % ） was significantly increased （ P 〈 0. 05 ） and the apoptosis rate （ 8. 51% vs. 14. 42% ） was sig- nificantly reduced in RvD1 ± LPS gr

关 键 词：消退素D1 脂多糖 小胶质细胞 炎症 

分 类 号：R96[医药卫生—药理学]