骨髓间充质干细胞联合超短波对大鼠脊髓损伤后早期AQP-4和GAP-43的影响  

Effects of bone marrow stromal cells transplantation combined with ultrashort wave therapy on the expression of AQP-4 and GAP-43 after spinal cord injury in rats

作　　者：孙师[1] 万峪岑 赵利娜[1] 尹艳梅[1] 陆宇[2] 冯智萍 周禹鑫[1] 张立新[1] 张志强[1] 

机构地区：[1]中国医科大学附属盛京医院,辽宁沈阳110022 [2]辽宁省人民医院

出　　处：《中国康复医学杂志》2016年第6期625-631,共7页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金青年基金资助项目(81101462)

摘　　要：目的:探讨骨髓间充质干细胞(bone marrow stromal cells,BMSCs)联合超短波(ultrashort wave,USW)治疗对大鼠脊髓损伤(spinal cord injury,SCI)后功能恢复、水通道蛋白4(aquaporin-4,AQP-4)和生长相关蛋白43(growth associated protein 43,GAP-43)表达的影响。方法:30只雌性SD大鼠,随机分为5组:sham组、control组、USW组、BMSCs组、USW+BMSCs联合治疗组,采用改良Allen's法制作大鼠SCI模型,术后1d、1周、2周、3周、4周用BBB评分法评价后肢运动功能的恢复情况。术后4周取材行AQP-4和GAP-43的免疫组化染色,并行阳性细胞计数,进行比较分析。结果:术后4周BBB评分各组比较有显著性意义,USW组、BMSCs组、USW+BMSCs组与control组比较P<0.001、P>0.05、P<0.001。USW组、BMSCs组、USW+BMSCs组AQP-4表达水平明显低于control组,有显著性意义(P<0.001、P<0.05、P<0.001)。GAP-43的表达上,BMSCs组、USW+BMSCs组明显多于control组及USW组,分别比较均有显著性意义(P<0.001)。结论:单纯USW治疗即可明显改善SCI后神经功能;单纯USW治疗及BMSCs移植治疗均可减轻水肿,以USW作用最为显著;而在促进轴突再生方面,BMSCs组意义更为明显。二者联合治疗在促进功能恢复上并未显现出协同作用,但是在减轻水肿和促进轴突生长方面有协同作用。Objective： To investigate the effects of bone marrow stromal cells（BMSCs）transplantation combined with low dose ultrashort wave（USW） radiation on the functional recovery and the expression of aquaporin-4（AQP-4） and growth associated protein 43（GAP-43） after spinal cord injury（SCI） in rats . Method： Thirty female Sprague-Dawley rats were randomly divided into five groups： sham-operated group, as well as control group,USW group, BMSCs group and USW＋BMSCs group that were subjected to spinal cord contusion.Basso-Beattie-Bresnahan （BBB） tests were performed before the operation and at ld, lw,2w,3w,4w after SCI. 4 weeks later, animals were sacrificed to make paraffin section.Immunohistochemical staining was performed to observe the expression of the AQP-4 and GAP-43. Result：BBB scores were higher in the USW, BMSCs and USW＋BMSCs groups than in the control group.The BBB scores in the USW and USW＋BMSCs groups increased significantly compared to that in the control group （P〈0.001 and P〈0.001, respectively）.All of treatment groups had lower AQP-4 expression than the control group （P〈0.001,P〈0.05 and P〈0.001 respectively）.Compared with the control and USW groups, the expres- sions of GAP-43 in BMSCs and USW＋BMSCs groups were higher significantly （P〈0.001, respectively）. Conclusion：The results indicated that USW radiation alone can obviously improve neural functional recovery after SCI .The USW radiation and BMSCs transplantation can alleviate the edema after SCI and USW radiation is more effective.But BMSCs transplantation can promote axon regeneration directly. The combination therapy showed a synergistic action on alleviating the edema and promoting axon regeneration, not but on promoting functional recovery.

关 键 词：脊髓损伤 骨髓间充质干细胞 超短波 水通道蛋白4 生长相关蛋白43 

分 类 号：R651.2[医药卫生—外科学]