电针联合游泳训练对脊髓全横断大鼠脊髓神经营养因子-3及血清谷丙转氨酶、谷草转氨酶的影响  

The effect of combining electroacupuncture and swimming training on NT-3,ALT and AST of rat after spinal cord transection

作　　者：杨燕兴 李灵玲[1] 刘鹏民[1] 王良[1] 周达岸[1] 

机构地区：[1]辽宁医学院附属第三医院,锦州121000

出　　处：《中国康复医学杂志》2016年第6期641-646,共6页Chinese Journal of Rehabilitation Medicine

基　　金：辽宁医学院校长基金--奥鸿博泽研究生科研创新基金(AH2014016)

摘　　要：目的:探讨电针联合游泳训练对脊髓全横断大鼠脊髓神经营养因子-3(NT-3)及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)的影响。方法:雌性成年SD大鼠180只,随机分为6个组(正常组,假手术组,损伤组,游泳组,电针组,联合组),每组30只。制作脊髓T10全横断损伤模型。术后第5天开始电针及游泳训练(电针每天30min,电流1m A,频率为2/100Hz,波型为疏密波。游泳第1周游一组,以后每周加一组,一组时间为4min。每周治疗7次)。对照组不进行治疗。术后7d、14d、21d、28d、35d进行运动功能评估(BBB评分),治疗结束时取材。用免疫组化及生化来检测相关因子的表达。结果:(1)各治疗组的运动功能评分较损伤组均有提高(除第1周(P>0.05)),并随时间的延长效果更为明显;在各干预组中,联合组运动功能的恢复最为突出(P<0.01)。(2)治疗组大鼠脊髓损伤区NT-3表达较损伤组均有提高(P<0.05),并随时间的延长而提高,治疗组之间联合组NT-3的表达更为突出(P<0.01)。(3)各治疗组中血清ALT、AST的表达水平较损伤组均有下降(P<0.05),联合组下降最为明显(P<0.01)。结论:游泳训练及电针能够促进脊髓损伤后运动功能的恢复,将两者联合应用效果更为明显。Objective： To study the effect of combining electroacupuncture and swimming training on NT-3 and ALT and AST of rat after spinal cord transection Method： 180 adult female SD rats were randomly divided into the normal group（30）, sham-operated group （30）, injured group（30）, swimming group（30）, electroacupuncture group（30）, and combination group （30）. T10 spinal cord transection performed on last 4 groups. Post operation day 5 began the electric acupuncture courtes periodes current, 30 minutes per day） and swimming training（the first week only 4 minutes a session, 7 times a week, one session more per week later until 5 sessions per day） for the last 3 groups. The other 2 groups accepted no treatment. Result： （1）The movement function of treated groups was better than tmtreated injured group（P 〉 0.05）, the lon- ger the treatment the better the improvement. The movement function index of the combination group increased more than electroacupuncture or swimming training separately（P〈 0.01）. （2） The expression of NT-3 of the treated groups increased more than the injured group （P〉 0.05）, and the combination group was better than the other two treated groups （P〈0.01）. （3） Compared with the injured group, the expression of serum ALT and AST levels decreased in treated groups （P〉 0.05）, and the combination group fell the most among the 3 treated groups （P 〈 0.01）. Conclusion： Swimming training and electroacupuncture can promote the recovery of movement function after spinal cord transection, and the effect is more apparent if combine the two.

关 键 词：督脉电针 游泳训练 脊髓全横断 神经营养因子-3 丙转氨酶 谷草转氨酶 

分 类 号：R245[医药卫生—针灸推拿学]