钙黄绿素-Cu^(2+)体系荧光恢复测定水样中H_2S  

Determination of H_2S in water sample by the fluorescence recovery of Calcein-Cu(Ⅱ) system

作　　者：张珊珊[1] 陈怡[1] 侯俊峰 李新霞[3] 关明[1] 

机构地区：[1]新疆师范大学化学化工学院,乌鲁木齐830054 [2]新疆阿克苏第十二中学,阿克苏843000 [3]新疆医科大学药学院,乌鲁木齐830000

出　　处：《分析试验室》2016年第6期645-647,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(81260486);新疆研究生科技创新项目基金(XJGRI2015107)资助

摘　　要：建立了钙黄绿素-Cu^(2+)体系荧光恢复法测定水样中痕量H_2S的新方法。在p H8.0的Na_2HPO_4-Na H_2PO_4缓冲溶液中,以472 nm为激发波长,510 nm为发射波长,运用标准曲线法测定水样中的H_2S含量,并进行精密度、加标回收率及稳定性实验的评价。线性范围为3.2×10^(-6)~2.3×10^(-5)mol/L,相关系数r为0.9978,检出限为1.1×10^(-6)mol/L,RSD为1.7%,平均回收率为106.9%。To establish a new effective method for determination of trace hydrogen sulfide in water sample by the fluorescence recovery of Calcein-Cu（ II） system.In Na_2HPO_4- Na H_2PO_4（ pH8） buffer,λex= 472 nm,λem=510 nm.H_2S in water sample was determined by the standard curve method.Precision and stability experiment,and the recovery experiment were all made at the same time.The linear range is from 3.2 × 10^（-6）mol/L to 2.3 ×10^（-5）mol/L,（ r = 0.9978）,the limit of detection is 1.1 × 10^（-6）mol/L,RSD = 1.7%,and the average recovery is 106.9%.Combining the high sensitivity of fluorescence with good stability of Calcein-Cu（ II） system,the developed fluorescent method is simple,sensitive,rapid,stable and has wide linear range for determining trace amounts of H_2S in water samples.

关 键 词：荧光恢复 钙黄绿素 硫化氢 

分 类 号：O657.3[理学—分析化学]