银(Ⅲ)配合物-鲁米诺化学发光体系测定盐酸士的宁  

Determination of strychnine by flow-injection chemiluminescence system of luminol and Ag( Ⅲ) complex

作　　者：刘菲[1] 林华萍[2] 韩旭[3] 李楠[2] 李享[2] 康维钧[2] 

机构地区：[1]河北中医学院基础医学院,石家庄050200 [2]河北医科大学公共卫生学院,石家庄050017 [3]河北省疾病预防控制中心,石家庄050000

出　　处：《分析试验室》2016年第6期664-667,共4页Chinese Journal of Analysis Laboratory

基　　金：河北省高等学校科学技术研究项目(Z2014030);河北省食品药品监督管理局科技项目(PT2014054);河北省研究生创新资助项目资助

摘　　要：建立了测定药物中盐酸士的宁的流动注射化学发光分析方法。盐酸士的宁能够显著抑制鲁米诺-Ag(Ⅲ)配合物体系的化学发光作用,且抑制强度与药物的浓度在一定范围内呈良好的线性关系。采用正交设计优化实验条件后盐酸士的宁的最低检出限为2.5×10^(-7)mol/L。线性范围为5.0×10^(-7)~1×10^(-5)mol/L和1.0×10^(-5)~1×10^(-4)mol/L,回归方程分别为△I=68c+16,相关系数r=0.997;△I=233c+518,相关系数r=0.990。对1×10^(-5)mol/L的盐酸士的宁连续进行平行测定11次,相对标准偏差为3.4%,加标回收率在85.4%~101.2%之间。并且应用高效液相色谱法对样品进行了验证,实验结果与化学发光法相符。A method of flow-injection chemiluminescence for determination of strychnine was developed.In alkaline medium,the intensity of chemiluminescence produced by the reaction of luminol and Ag（ Ⅲ） complex could be depressed by strychnine.Orthogonal experimental design was applied to decide the optimal parameters.Under the optimum experimental condition,the linear equation of the method were △I = 68 c ＋ 16（ 5.0 × 10^（- 7）～ 1 × 10^（- 5）mol/L） and △I = 233 c ＋ 518（ 1.0 × 10^（- 5）～ 1 × 10^（- 4）mol/L）,r = 0.997 and 0.990.The recoveries of standard addition were in the rang of 85.4% ～101.2%,with the relative standard deviation of3.4%,and the detection limit was 2.5 × 10^（- 7）mol/L based on signal-to-noise ratio of 3： 1.The results were validated by HPLC.

关 键 词：鲁米诺 Ag(Ⅲ)配合物 化学发光 盐酸士的宁 

分 类 号：R284[医药卫生—中药学]