基因A型鸭甲肝病毒弱毒株免疫雏鸭的8种细胞因子mRNA变化分析  

mRNA Change Analysis of Eight Cytokines in Ducklings Stimulated by Attenuated Duck Hepatitis A Virus Genotype A MY Strain

作　　者：张焕容[1] 张冰[1] 王栋[1] 岳华[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《畜牧兽医学报》2016年第6期1215-1221,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中央高校基本科研业务费专项资金项目(2014NZYQN58);国家民委科技项目(14XNZ025)

摘　　要：为研究基因A型鸭甲肝病毒(DHAV-A)MY弱毒株对雏鸭细胞因子表达的影响,阐明细胞因子在DHAV-A弱毒疫苗免疫中的作用,利用荧光定量RT-PCR(RRT-PCR)方法,检测DHAV-A MY弱毒株接种1日龄SPF雏鸭后0.25、0.5、1、2、3和5d共6个时间点肝、脾和脑组织中IFN-α、IFN-β、IFN-γ,以及IL-1β、IL-2、IL-6、IL-8和TNF-α共8种细胞因子的mRNA转录水平,并结合各时间点DHAV-A的荷载量进行分析。结果表明:肝和脑组织中2d时间点以及脾组织中3d时间点病毒荷载量达到最高;三种组织中,8种细胞因子的转录水平均随着病毒荷载量的增加而升高,且在各组织病毒荷载量最高时间点,8种细胞因子的转录水平均极显著升高(P<0.01),结果证明DHAV-A MY弱毒株可刺激雏鸭抗病毒细胞因子IFN-α、IFN-β、IFN-γ和TNF-α以及炎性细胞因子IL-1β、IL-2、IL-6和IL-8mRNA的转录,本研究丰富了DHAV-A MY弱毒株的免疫机制。In order to explore the roles of cytokines responding to attenuated duck hepatitis A virus genotype A（DHAV-A）vaccine immunization,the effects of attenuated DHAV-A MY strain on cytokine transcription in ducklings were analyzed.The mRNA transcription levels of 8cytokines,such as IFN-α,IFN-β,IFN-γand IL-1β,IL-2,IL-6,IL-8,and TNF-α,were determined by RRT-PCR in liver,spleen and brain of ducklings at different time points of 0.25 0.5,1,2,3and 5days post attenuated DHAV-A MY strain injecting 1-day-old SPF ducklings,and the virus loads of DHAV-A MY strain in liver,spleen and brain were also detected by RRT-PCR.The results indicated that the virus load reached the highest level in liver and brain at 2dtime point,and at 3d time point in spleen post attenuated DHAV-A MY strain injection.The mRNA transcription levels of 8cytokines in liver,spleen and brain up-regulated very significantly at the corresponding time points when the virus load reached the highest level post DHAV-A MY strain injection（P〈0.01）.The results indicated that attenuated DHAV-A MY strain could stimulate the transcription of anti-viral cytokines IFN-α,IFN-β,IFN-γand TNF-α,as well as proinflammatory cytokines IL-1β,IL-2,IL-6and IL-8,our results enrich the immunization mechanism of DHAV-A MY strain.

关 键 词：基因A型鸭甲肝病毒 弱毒株 细胞因子 荧光定量RT-PCR 

分 类 号：S855.3[农业科学—临床兽医学]