抗生素利福平对萼花臂尾轮虫生命表参数的影响  

Effects of rifampicin on life table demography of Brachionus calyciflorus under different Scenedesmus obliquus densities

作　　者：翟盼 温新利[1] 陈治文[1] 赵政[1] 李海洋[1] 席贻龙[1] 

机构地区：[1]安徽师范大学生命科学学院安徽省高校生物环境与生态安全省级重点实验室皖江城市带退化生态系统的恢复与重建安徽省协同创新中心,安徽芜湖241000

出　　处：《中国环境科学》2016年第6期1886-1894,共9页China Environmental Science

基　　金：国家自然科学基金(31470015);安徽省自然科学基金项目(1208085QC59);重要生物资源的保护与利用研究安徽省重点实验室专项基金;安徽省高等学校大学生创新创业训练计划专项基金

摘　　要：以萼花臂尾轮虫（Brachionus calyciflorus）为受试动物,研究了在不同斜生栅藻（Scenedesmus obliquus）密度（1.0×10~6,2.0×10~6,4.0×10~6个/m L）下,不同浓度（2.0,4.0,6.0,8.0,10.0μg/m L）的利福平溶液对萼花臂尾轮虫实验种群增长参数的影响.结果表明,与空白对照组及助溶剂对照组相比,1.0×10~6个/m L藻密度下,各浓度的利福平显著提高了轮虫的后代混交率;2.0×10~6个/m L藻密度下,2.0,4.0,10.0μg/m L的利福平显著提高了轮虫的內禀增长率,各浓度的利福平均提高了轮虫的后代混交率;4.0×10~6个/m L藻密度下,各浓度的利福平均显著提高了轮虫的內禀增长率和后代混交率.藻密度为1.0×10~6个/m L时,利福平浓度与轮虫的生命期望、世代时间、平均寿命和后代混交率间具有显著的剂量-效应关系;藻密度为2.0×10~6个/m L时,利福平浓度与轮虫的后代混交率间具有显著的剂量-效应关系;藻密度为4.0×10~6个/m L时,利福平浓度与轮虫的世代时间、净生殖率、內禀增长率和后代混交率间有显著的剂量-效应关系.By means of life-table technique, the effects of rifampicin at 2.0, 4.0, 6.0, 8.0, and 10.0μg/mL on the population growth parameters ofBrachionuscalyciflorus under threeScenedesmus obliquusdensities （1.0＆#215;106, 2.0＆#215;106and 4.0＆#215;106cells/mL） were studied. The results showed that compared to the controls, rifampicin at all the test concentrations obviously promoted the proportion of sexual offspring under the algal density of 1.0＆#215;106cells/mL （P＆lt;0.05）. When algal density was 2.0＆#215;106cells/mL, rifampicin at 2.0, 4.0 and 10.0μg/mL increased the intrinsic rate of population increase （P＆lt;0.05）, and rifampicin at all the test concentrations also increased the proportion of sexual offspring. When algal density was 4.0＆#215;106cells/mL, rifampicin at all the test concentrations obviously promoted the intrinsic rate of population increase and the proportion of sexual offspring （P＆lt;0.05）. There were significant dose-effect relationships between rifampicin concentration and the life expectancy at hatching, the generation time, the average lifespan, and the average lifespan of the rotifers fed on the 1.0＆#215;106cells/mL of algae. When algae density was 2.0＆#215;106cells/mL, there were significant dose-effect relationships between the concentration of rifampicin and the proportion of sexual offspring. When algal density was 4.0＆#215;106cells/mL, there were significant dose-effect relationships between the rifampicin concentration and the generation time, the net reproductive rate, the intrinsic rate of population increase as well as the proportion of sexual offspring.

关 键 词：利福平 斜生栅藻密度 萼花臂尾轮虫 生命表参数 

分 类 号：X503.225[环境科学与工程—环境工程]