鸡源CD154分子功能区的表达  

Expression of functional domain of chicken CD154

作　　者：段雨路 韩春燕[1] 么帅[1] 高玉龙[1] 祁小乐[1] 高立[1] 李凯[1] 刘长军[1] 张艳萍[1] 高宏雷[1] 王永强[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽免疫抑制病研究团队,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2016年第6期493-495,499,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31430087);国家现代农业产业技术体系建设专项基金(nycytx-42-G3-01);兽医生物技术国家重点实验室自主研究课题(SKLVBP2015009)

摘　　要：为表达鸡源CD154分子的功能区,本研究根据Gen Bank中鸡CD154的参考序列克隆其编码区。通过融合PCR将CD154的C端与小鼠CD8α蛋白的N端串联,并连接信号肽以利于分泌表达,将重组质粒转染293T细胞,检测其表达情况。结果显示,融合蛋白正确表达,表达量随时间的延长而增加,并且能够分泌到细胞外。鸡CD154分子功能区的表达,为更好地在体外研究传染性法氏病病毒感染B淋巴细胞奠定了很好的基础。In order to express the fimctional domain of chicken CD154 in a secretory way. The open reading frame of chicken CD154 was amplified based on the reference sequence in GenBank. The tandem protein was comprised with the N-terminal of mouse CD8a and C-terminal of chicken CD154. Additionally, the signal peptide was fused to induce secretory expression of fusion protein. The results showed that the fusion protein expressed successfully and the amount of fusion protein increased at 48 hours post transfection in 293T cells. It is important that the fusion protein can secrete into medium from cells. The expression of functional domain of chicken CD154 supports the investigation of infection mechanism in different strains of infectious bursal disease virus in bursal B cells in vitro.

关 键 词：鸡 CD154分子 表达 传染性法氏囊病病毒 

分 类 号：S852.4[农业科学—基础兽医学]