油茶柠檬酸合成酶(CS)基因的克隆和表达分析  

Cloning and Expression Analysis of Citrate Synthase(CS)Gene in Camellia oleifera

作　　者：叶思诚[1] 姚小华[1] 王开良[1] 林萍[1] 龚洪恩[1] 卓仁英[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400

出　　处：《植物研究》2016年第4期556-564,共9页Bulletin of Botanical Research

基　　金：浙江省重大农业专项"油茶高产优质新品种选育及示范"(2012C12908)~~

摘　　要：采用RT-PCR技术从油茶中分离出一个柠檬酸合成酶基因,该基因的c DNA全长1 416 bp,编码471个氨基酸,推导的蛋白分子量为52.74 k D,理论等电点(PI)为6.95。同源比对显示其与其他植物的CS蛋白序列高度同源,将该基因命名为Co CS(Gen Bank登录号:KU161147)。系统进化树分析表明油茶Co CS与杜鹃和葡萄的CS蛋白的亲缘关系较近。荧光定量PCR分析结果表明,油茶受到低磷胁迫后根系Co CS基因的表达受到低磷诱导,表达量呈现先升高后降低的趋势;不同油茶品种不同组织(根、茎、叶)中的Co CS基因在不用磷处理下的表达模式不同。A citrate synthase（CS） gene were isolated from Camellia oleifera by RT-PCR. The full-length cDNA of the CS is 1 416 bp in size, encodeding a deduced polypetide of 471 amino acids with estimated molecular weight of 52.74 kD and theoretical isoelectric point of 6.95. Homologous alignment showed that the deduced protein had high identities with the CS proteins of other plants, therefore the gene was named as CoCS （ Genbank No. KU161147）. By phylogenetic tree analysis, CoCS had close genetic relationships with Rhododendron micranthum and Vitis vinifera. By qRT-PCR, the expression of CoCS in root was induced by phosphate deficiency and increased at first and decreased subsequently. The expression patterns of CoCS were different among different tissues and different cuhivars.

关 键 词：油茶 柠檬酸合成酶 低磷 基因克隆 表达分析 

分 类 号：S794.4[农业科学—林木遗传育种]