异补骨脂查尔酮抗哮喘作用的实验研究  

The anti- asthmatic effect of isobavachalcone: an experimental study

作　　者：梁正[1] 郝文龙[2] 孙雪英[1] 杨彧[1] 

机构地区：[1]成都中医药大学,四川成都610075 [2]内蒙古集宁师范学院,内蒙古集宁012000

出　　处：《湖南中医杂志》2016年第5期177-179,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：成都中医药大学科技发展基金资助项目(编号:ZRQN1417)

摘　　要：目的:探讨异补骨脂查尔酮在实验动物体内抗哮喘的作用。方法:将27只野生型雌性BALB/c小鼠随机分为正常组(7只)、哮喘对照组(10只)、给药组(10只),采用卵清白蛋白(OVA)致敏诱导哮喘模型,给药组灌胃具有抑制IL-4分泌的化合物异补骨脂查尔酮,连续1周。检测给药后模型动物体内脾脏CD4^+细胞及血清中IL-4、IFN-g水平的变化。结果:与哮喘对照组相比,给药组体内脾脏CD4^+细胞中IL-4的产生减少(P<0.05),IFN-g表达无明显差异,血清中IL-4的表达降低(P<0.05),血清中IFN-g的表达增高(P<0.01)。结论:异补骨脂查尔酮能够抑制哮喘小鼠体内IL-4的表达,初步证实有一定的抗哮喘活性,其具有开发成为治疗哮喘药物的潜力。Objective： To investigate the anti- asthmatic effect of isobavachalcone in experimental animals. Methods： A total of 27 wild- type female BALB / c mice were randomly divided into normal group（ 7mice）,asthma control group（ 10 mice）,and medication group（ 10 mice）. Ovalbumin sensitization was used to establish the model of asthma. The mice in the medication group were given isobavachalcone which inhibited the secretion of interleukin- 4（ IL- 4） by gavage for 1 week continuously. The changes in spleen CD4-^＋cells and serum levels of IL-4 and interferon- α（ IFN- g） in model animals were measured. Results： Compared with the asthma control group,the medication group showed a significant reduction in the secretion of IL- 4 in CD4-^＋cells（ P〈0. 05）,while IFN- g showed no significant difference between the two groups; the medication group showed a significant reduction in the serum level of IL- 4（ P〈0. 05） and a significant increase in the serum level of IFN- g（ P〈0. 01）.Conclusion： Isobavachalcone can inhibit the expression of IL- 4 in asthmatic mice and is proven to have the anti-asthmatic effect,and therefore,it can be used in the treatment of asthma.

关 键 词：哮喘 异补骨脂查尔酮 IL-4 IFN-Α 卵清白蛋白 实验研究 

分 类 号：R259.622.5[医药卫生—中西医结合]