氙气减轻庆大霉素诱导的大鼠急性肾损伤  

Xenon reduces gentamicin-induced acute kidney injury in rats

作　　者：贾平[1] 丁小强[1] 

机构地区：[1]复旦大学附属中山医院肾内科,上海200032

出　　处：《中国临床医学》2016年第2期136-138,共3页Chinese Journal of Clinical Medicine

摘　　要：目的:观察氙气对庆大霉素肾毒性的预防作用。方法:选择雄性Wistar大鼠48只,随机分成4组。模型组:腹腔注射硫酸庆大霉素100mg/(kg·d),连续7d;对照组:腹腔注射等体积的0.9%氯化钠液;氙气处理组:先予70%氙气+30%氧气暴露2h,24h后予庆大霉素100mg/(kg·d)腹腔注射,连续7d,此间分别于第2、4、6天给药前再次给予70%氙气+30%氧气暴露30min;氮气处理组:作为氙气处理的对照,将氙气改为70%氮气,方法同氙气处理组。分别于给药结束后第2、4天检测各组大鼠肾功能、肾脏形态学及氧化应激指标。结果:与对照组相比,模型组与氮气处理组大鼠在给药结束后第2、4天,肾功能明显减退,表现为血肌酐与尿素氮水平明显升高;肾小管损伤指数明显增大;氧化应激指标丙二醛浓度也高于对照组,超氧化物歧化酶活性下降(P<0.01)。而氙气处理组与模型组、氮气处理组相比,肾损伤程度明显减轻,表现为血肌酐、尿素氮浓度降低,肾小管损伤指数减小,肾组织丙二醛浓度也降低,超氧化物歧化酶活性升高(P<0.01)。结论:间断氙气干预可以减轻庆大霉素诱导的大鼠急性肾损伤。Objective： To observe the effect of xenon exposure on gentamicin-induced acute kidney injury. Methods.. 48 male Wistar rats were randomly divided into four groups as follows., the model group, where rats were given intraperitoneal （i. p. ） injection of gentamicin sulfate solution 100 mg/（kg · d） for 7 days; the control group, where rats were intraperitoneally administrated physiological saline （0. 9 % ） for 7 days the N2 gentamicin group （N2 - gent） and the Xenon＋ gentamicin group （Xeq-gent）, where rats were pretreated with 70% nitrogen or 70% xenon balanced with 30% oxygen for 2 h, followed by 7 days of gentamicin treatment initiated 24 h later, and repeatedly treated with 70% nitrogen or 70% xenon for 30 min on day 2, 4, and 6 during the 7-day administration of gentamicin. Renal function, renal morphology and, oxidative stress indexes were collected respectively on day 2, 4 after the last gentamicin injection for assay （n^6 at each time point per group）, serum creatinine （Scr） and blood urea nitrogen （BUN） were examined. The kidney tissue was stained by H-E. Renal malondialdehyde （MDA） and renal superoxide dismutase （SOD） activity were measured. Results： Compared with the control group, Scr and BUN levels were significantly increased on day 2, 4 after last gentamicin injection in the model group and N2 q-gent group, renal tubular damage index increased significantly, MDA concentration, the oxidative stress index, were also higher than that of the control group, and the activity of SOD decreased, all showing obvious decline in renal function（P〈0. 01）. Compared with the model group and the N2 q-gent group, the degree of renal injury was significantly reduced in the Xed-gent group： Scr and BUN concentration decreased, renal tubular damage index decreased, the MDA concentration in renal tissue decreased, and SOD activity increased significantly（P〈0. 01）. Conclusions： Xenon treatment can significantly reduce gentamicin-induced acute kidney injury in ra

关 键 词：氙气 庆大霉素 急性肾损伤 氧化应激 

分 类 号：R692[医药卫生—泌尿科学]