不同品系忍冬外观性状与内在品质比较分析  

Comparative Analysis of Different Strains of Lonicera japonica on Appearance and Internal Quality

作　　者：边丽华[1,2] 周洁[2,3] 于德强 刘伟[2] 耿岩玲[2] 王晓[2] 李圣波 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省中药质量控制技术重点实验室/山东省分析测试中心,山东济南250014 [3]道地药材国家重点实验室/中国中医科学院中药资源中心,北京100700 [4]威海文登区妇幼保健院,山东威海264400 [5]山东亚特生态技术有限公司,山东临沂276000

出　　处：《中药材》2016年第3期504-509,共6页Journal of Chinese Medicinal Materials

基　　金：山东省自主创新专项(2013CXC20401);中央本级重大增减支项目子课题(20603020124)

摘　　要：目的:为忍冬种质资源评价及优良品系选育提供基础资料。方法:采用形态分类学方法对不同品系忍冬进行观察、描述和初步分类;采用高效液相色谱法检测不同品系忍冬花蕾和叶片中新绿原酸、绿原酸、隐绿原酸、木犀草苷、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸的含量;利用SPSS 17.0软件进行数据分析。结果:不同品系忍冬在叶色、叶形及花蕾大小和颜色四个方面表现出显著差异;品系YTQL-01、ITA-01花蕾中新绿原酸、绿原酸和3,5-二咖啡酰奎宁酸等的含量较高;不同品系忍冬外观性状与有效成分含量之间未表现出显著相关性。结论:不同品系忍冬在外观性状和内在品质上均表现出丰富的遗传多样性。Objective： To provide the reference for germplasm identification and breeding of Lonicera japonica. Methods： Morphological taxonomy was used to observe,describe and preliminary classify different strains of Lonicera japonica. HPLC was used to determine the content of 5-caffeoylquinic acid,chlorogenic acid,cryptochlorogenic acid,luteolin,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in the buds and leaves; SPSS 17. 0 was used to analyze the data. Results： The differences between different strains of Lonicera japonica were on four aspects（ leaf color,leaf shape,bud size and color）. The buds of strain YTQL-01 and ITA-01 contained higher content of 5-caffeoylquinic acid,chlorogenic acid and 3,5-dicaffeoylquinic acid. There was no significant correlation between appearance and internal quality of different Lonicera japonica strains. Conclusions： Genetic diversity exists between different strains of Lonicera japonica according to appearance and internal quality.

关 键 词：忍冬 品系 外观性状 内在品质 

分 类 号：R282.71[医药卫生—中药学]