益髓理血饮对二甲基苯蒽诱导骨髓增生异常综合征模型大鼠骨髓病态造血的影响及机制  

Effects and Mechanism of Yisui Lixue Decoction on Dyshaematopoiesis of Marrow Cell in Model Rats with Myelodysplastic Syndrome Induced by Dimethyl Benzanthracene

作　　者：黄玉静[1] 熊德上[1] 徐酉华[2] 于洁[2] 宪莹[2] 蔡锷 

机构地区：[1]重庆医科大学中医药学院,重庆401331 [2]重庆医科大学附属儿童医院,重庆400014 [3]重庆将军血液病研究所,重庆400041

出　　处：《中药材》2016年第3期619-624,共6页Journal of Chinese Medicinal Materials

基　　金：重庆市卫生局中医药科技项目(2011-2-8)

摘　　要：目的:探讨中药复方益髓理血饮对二甲基苯蒽(DMBA)诱导的骨髓增生异常综合征(MDS)模型大鼠骨髓病态造血的改善作用及其机制。方法:将清洁级雄性SD大鼠分为正常对照组、模型组、复方皂矾丸阳性组及益髓理血饮低、高剂量组,每组12只。用DMBA诱导建立大鼠MDS模型,于造模第14天开始分组给药,连续30 d。给药后第31天处死各组大鼠,检测各组大鼠骨髓增生程度、病态造血,血清IL-3、TNF-α含量,股骨CD34表达及原始细胞所占比。结果:与正常对照组比较,模型组骨髓增生程度和骨髓病态造血明显,各给药组骨髓增生和病态造血明显改善;模型组血清IL-3含量显著降低(P<0.01),TNF-α含量显著升高(P<0.01),治疗后各给药组IL-3含量显著升高(P<0.05),TNF-α含量降低(P<0.05);模型组股骨CD34及髓CD45阳性表达(P<0.01),治疗后股骨CD34表达和原始细胞比例均显著降低(P<0.05)。以上改善均以高剂量组最为明显。结论:益髓理血饮治疗骨髓增生异常综合征能改善骨髓增生程度和病态造血,升高血清IL-3含量,降低血清TNF-α含量,减少股骨CD34阳性表达,降低股骨原始细胞比例。Objective： To investigate the effects and mechanism of Yisui Lixue decoction on dyshaematopoiesis of marrow cell in model rat with myelodysplastic syndrome（ MDS） induced by dimethyl benzanthracene（ DMBA）. Methods： The model rats with MDS were induced by the chemical mutagens DMBA,which randomly divided into normal control group,physiological saline model group,compound Zaofan pill group,low dose group and high dose group of Yisui Lixue decoction,with 12 rats in each group. The rats were treated with different drugs for one month from the 14 th day,and executed on the 31 th day. The degree of bone marrow hyperplasia,dyshaematopoiesis,IL-3 and TNF-α in serum,the expression of CD34,and the proportion of the original cells were measured in the experimental group. Results： Compared with the normal control group,the degree of bone marrow hyperplasia was hyperactive and dyshaematopoiesis was more obvious in the physiological saline model group; and in the treatment group were improved,especially in the high dose group of Yisui Lixue decoction. Compared with the normal control group,the content of IL-3 in serum was decreased and TNF-α was increased（ P 〈0. 01） in the physiological saline model group; the content of IL-3 was increased（ P 〈0. 05） and THF-α was decreased（ P 〈0. 05） in the treatment groups,the effects were more obvious（ P 〈0. 01） in the high dose group of Yisui Lixue decoction. Compared with the normal control group,the positive expression of CD34 and CD45 were significantly increased（ P 〈0. 01） in the physiological saline model group,and those in treatment groups were decreased（ P 〈0. 05）,especially in the high dose group of Yisui Lixue decoction（ P 〈0. 01）. Conclusion： Yisui Lixue decoction can improve the degree of bone marrow hyperplasia,dyshaematopoiesis,elevate the expression of IL-3,reduce the expression of TNF-α and CD34 and CD45.

关 键 词：骨髓增生异常综合征 益髓理血饮 骨髓病态造血 IL-3 TNF-α CD34 

分 类 号：R285.5[医药卫生—中药学]