模拟微重力对猕猴肺组织JNK/c-Jun和c-Fos信号通路的影响  

Effects of Simulated Microgravity on JNK/c-Jun/c-Fos Signaling Pathway in Lung of Rhesus Monkey

作　　者：陈杨[1] 王萍[1] 许崇玉 蔡艺灵[1] 马华松[1] 

机构地区：[1]解放军306医院呼吸与重症医学科,北京100101 [2]安徽医科大学解放军第306临床学院,北京100101

出　　处：《航空航天医学杂志》2016年第5期537-540,共4页Journal of Aerospace medicine

基　　金：306医院院级青年课题(编号:14QN04)

摘　　要：目的探讨模拟微重力对猕猴肺组织结构和JNK/c-Jun和c-Fos信号通路的影响。方法在特殊装置上采用猕猴头低位-10°模型模拟微重力,15只猕猴分为3组,每组5只:对照组(A组)、模拟微重力组(B组)、模拟微重力后恢复组(C组)。HE染色观察各组猕猴肺组织大体结构,Western blotting检测肺组织JNK、cJun、c-Fos及磷酸化表达水平。结果 HE显示,与A组相比,B组和C组猕猴肺组织可见肺泡间隔不同程度的增宽,部分肺泡融合,肺间质内可见炎细胞浸润。C组病理改变较B组稍减轻。Western blotting结果提示,与A组相比,B组和C组JNK、c-Fos和p-c-Fos及p-c-Jun表达增多。结论长期处于微重力状态可引起猕猴肺组织损伤,肺组织中JNK、c-Fos磷酸化和c-Jun磷酸化表达增加,微重力激活JNK/c-Jun和c-Fos信号通路调控其下游特定基因的表达可能是肺损害的机制之一。Objective To investigate the effects of simulated microgravity on JNK/c- Jun/c- Fos cell signaling pathway change in lung of rhesus monkey. Methods Fifteen healthy young male rhesus monkeys were randomly divided into 3 groups： control group（ group A,n = 5）,6 weeks＇ simulated weightlessness group（ group B,n = 5） and recovery for 4 weeks after 6 weeks of simulated weightlessness group（ group C,n = 5）.- 10 degree head- down- tilt was used to simulated weightlessness. HE staining was used to observe the histopathological changes of the lung tissues. Western blot assay was performed to detect the levels of JNK,c- Jun,c- Fos and their phosphorylated levels. Results Compared with group A,histopathological examination revealed alveolar septal thickening,partial fusion of pulmonary alveoli and alveolar and interstitial lymphocytic infiltration in group B and C. And the pathological changes in group C were lighter than those in group B. The increased levels of JNK,c- Fos,p- c- Fos and p- c- Jun protein were observed in group B and C by western blotting. Conclusions Simulated microgravity can cause lung tissue damage and increase the levels of JNK,c- Fos,p- c- Fos and p- c- Jun protein in lung tissue of rhesus monkey. Microgravity may promote downstream genes expression by JNK/c- Jun/c- Fos cell signaling pathway which may be related to the damage of lung tissue in simulated microgravity.

关 键 词：模拟微重力 猕猴 肺组织 JNK/c-Jun/c-Fos信号通路 

分 类 号：R563[医药卫生—呼吸系统]