鹅掌楸组培苗专用菌剂筛选及其作用机理初探  

Screening of Microorganism Agents for Liriodendron chinense Tissue Culture Seedling and Preliminary Study on Its Mechanism

作　　者：谢越盛 李可[1] 王大成[1] 谢平[1] 郭坚华[1] 

机构地区：[1]南京农业大学植物保护学院/江苏省生物源农药工程中心/农作物生物灾害综合治理教育部重点实验室,南京210095

出　　处：《中国生物防治学报》2016年第3期332-341,共10页Chinese Journal of Biological Control

基　　金：国家自然科学基金(31171809);江苏省农业三新工程(SXGC[2014]305)

摘　　要：评价了不同菌剂对鹅掌楸组培苗移栽存活和生长势的影响,以筛选出鹅掌楸组培苗专用菌剂,并对其作用机理进行初步探讨。结果表明,微生物菌剂"宁盾"以及AM真菌GZ38处理组移栽存活率分别高达99.67%、96.12%,显著高于对照组,温室促生效果分别达53.71%、52.84%;且田间促生效果分别达26.56%、18.37%,存活率显著提高。机理研究表明,菌株GZ38能够较好地与鹅掌楸形成共生关系;"宁盾"、菌株GZ38不仅能够提高鹅掌楸的根系活力,还能够提高土壤中转化酶、脲酶、中性磷酸酶活性以及有效N、P、K的含量,促进其对N、P、K等营养元素吸收,达到促生效果;另外,菌剂处理后,鹅掌楸的膜损伤程度降低,保护酶活性有较大的提高,抵抗不良环境的能力显著增强。因此,选择微生物菌剂"宁盾"与AM真菌GZ38作为鹅掌楸组培苗专用微生物菌剂。This experiment is aiming to evaluate the impact of using microorganism agent ＂Nanjing Shield＂ and 13 strains of arbuscular mycorrhizal fungi （AMF） screened from rhizosphere of tobacco on Liriodendron chinense tissue culture seedling, by detecting the transplanting survival rate and growth-promoting effect. We tried to screen the best microorganism agent for the tissue culture seedling, as well as make clear the basis of its mechanism. The results showed that, the transplanting survival rates of ＂Nanjing Shield＂ and GZ38 as high as 99.67% respectively, were significantly higher than the control. Furthermore, the growth-promoting effect of Shield＂ and GZ38 treatments were 53.71%, 52.84% respectively in greenhouse, and that of GZ38 and 96.12% ＇Nanjing ＇Nanjing Shield＂ in the field were 26.56%, 18.37% severally. Further experiments showed that GZ38 could colonize at rhizosphere of seedling. GZ38 and ＂Nanjing Shield＂ could not only enhance the seedling＇s root activity and absorption of nutrient elements such as N, P, K, also could improve the contents of invertase, urease, neutral phosphatase and available N, P, K in soil. In addition, GZ38 and ＂Nanjing Shield＂ were able to reduce the contents of MDA and improve protective enzymes such as SOD, POD, CAT in seedlings to enhance resistance to adverse environment. Consequently, it is assumed that GZ38 and ＂Nanjing Shield＂ might be efficient microorganism agents as the solution of low transplanting survival rate and slowly growth when transplanting Liriodendron chinense tissue culture seedlings to the soil.

关 键 词：鹅掌楸组培苗 微生物菌剂 促生机理 

分 类 号：S475[农业科学—农业昆虫与害虫防治]