珠宝加工业苯接触作业人员XRCC1基因多态性与染色体损伤的关系  

Association between XRCC1 polymorphisms and chromosome damage in workers exposed to benzene in jewelcrafting industry

作　　者：黄丽静[1] 彭志敏[1] 张喜中[1] 尹强兵[1] 池洪城[1] 

机构地区：[1]深圳市罗湖区疾病预防控制中心职业卫生科,518020

出　　处：《中华劳动卫生职业病杂志》2016年第6期416-420,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：深圳市知识创新计划基础研究项目（JCYJ20130401153440214）

摘　　要：目的探讨X射线交叉互补修复基因1（XRCC1）基因多态性与珠宝加工业低浓度苯接触工人染色体损伤易感性的关系。方法于2013年1月至2014年12月，选取辖区内珠宝加工企业苯接触工人286名为研究对象。采用气相色谱法测定工作场所中苯浓度，采用胞质分裂阻滞微核试验分析研究对象的周围血染色体损伤水平，采用Sequenom技术检测XRCC1基因多态性位点。结果作业场所中苯的时间加权平均浓度（CTWA）为〈0．6-1．8mg／m^3，低于国家职业接触限值（6mg／m^3）；人群位点等位基因频率分布均符合遗传学Hardy—Weinberg平衡定律（P〉0．05）；研究对象年龄增长（RR=1．38，95％CI：1．06～3．75）和工龄增长（RR=1．45，95％CI：1.18～2．58）是微核率升高的危险因素；与野生纯合基因型比较，携带XRCC1 rs25487CT基因型的个体微核率升高的风险较高，差异有统计学意义（RR=1．51，95％C1：1．28．3．87，P〈0．05），携带XRCC1rs1799782AA基因型的个体微核率升高的风险较高，差异有统计学意义（RR=1．65，95％C1：1．30～3．12，P〈0．05），XRCC1rs25489基因多态与微核率无明显关联（P〉0．05）。结论低浓度苯暴露可能引起苯接触工人染色体损伤，XRCC1rs25487和XRCC1rs1799782基因多态可能与苯致染色体损伤有关联。Objective To investigate the association between the gene polymorphisms of the DNA damage repair gene X-ray repair cross-complementing gene 1 （XRCC1） and susceptibility to chromosome damage in workers exposed to low-concentration benzene in the jewelcrafting industry. Methods A total of 286 workers exposed to benzene in jewelcrafting enterprises were enrolled as study subjects from January 2013 to December 2014. Gas chromatography was used to measure benzene concentration in workplace, cytokinesisblock micronucleus test was used to analyze the level of chromosome damage in peripheral blood, and the Sequenom technique was used to determine the single nucleotide polymorphisms of XRCC1. Results The timeweighted average concentration of benzene in workplace was 〈0.6 -1.8 mg/m3, lower than the national occupational exposure limit （6 mg/m3）. The distribution of allele frequencies met the Hardy-Weinberg equilibrium in genetics （P〉0.05）. Increase in age （RR=1.38, 95%C1 1.06-3.75） and increase in working years （RR =1.45, 95% CI 1.18-2.58） were risk factors for the increase in micronucleus frequency. Compared with those with the wild-type homozygous genotype, the individuals with XRCC1 rs25487 CT genotype showed a significantly higher risk of increase in micronucleus frequency （RR=1.51, 95%CI 1.28-3.87, P〈0.05）, and the individuals with XRCC1 rs1799782 AA genotype also showed a significantly higher risk of increase in micronucleus frequency （RR=1.65, 95%CI 1.30-3.12, P〈0.05）. There was no clear association between XRCC1 rs25489 polymorphisms and micronucleus frequency （P〉0.05）. Conclusion Exposure to low- concentration benzene may cause chromosome damage in workers exposed to benzene, and the XRCC1 polymorphisms rs 25487 and rs1799782 may be associated with chromosome damage induced by benzene.

关 键 词：珠宝加工 苯 职业暴露 基因多态性 

分 类 号：R135[医药卫生—劳动卫生]