屎肠球菌和枯草芽孢杆菌混合培养条件的优化  

Study on Mixed Culture of Enterococcus faecium and Bacillus subtilis

作　　者：胡治铭 梁丛丛[1] 国洪旭[1] 邓先余[1] 林连兵[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《食品与生物技术学报》2016年第5期537-542,共6页Journal of Food Science and Biotechnology

基　　金：云南省科技创新强省项目(2014AB015)

摘　　要：以屎肠球菌和枯草芽孢杆菌为研究对象,以MRS培养基为基础培养基,采用单因素试验、Box-Behnken实验设计对屎肠球菌和枯草芽孢杆菌的混合培养进行优化。结果表明:初始p H、装液量和氮源对活菌数影响显著。最优条件为:初始p H 6.5、装液量50/500 m L、氮源用量35 g/L、接种比1∶12、转速180 r/min,在此条件下活菌数达到6.99×1010 CFU/m L,比优化前提高了5倍,屎肠球菌菌株和枯草芽孢杆菌菌株的活菌数分别达到4.58×1010 CFU/m L和2.41×1010CFU/m L。In order to investigate the effect of Enterococcus faecium and Bacillus subtilis mixed culture on their biomass,MRS medium,single factor experiment,Box-Behnken Design were adopted to optimize the mixed culture. Results showed that medium initial p H,liquid volume and nitrogen source had significant effects on the viable counts of the strains. The optimal culture conditions were as follows：initial medium p H 6.5,50 m L liquid medium in 500 m L flask with 180 rpm in shake,35 g/L of nitrogen source,the inoculation of Enterococcus faecium and Bacillus subtilis at a ratio of 1 ∶12. Under this conditions,the total viable counts reached to 6.99 ×1010CFU/m L,with 4.58 ×1010CFU/m L of Enterococcus faecium and 2.41 ×1010CFU/m L of Bacillus subtilis,which was five times higher than that of before optimization.

关 键 词：屎肠球菌 枯草芽孢杆菌 混合培养 

分 类 号：S816.7[农业科学—饲料科学]