复方前愈水煎液中多糖的纯化  

Optimization of Purification Process of Polysaccharide in Compound Qianyu Water Decoction

作　　者：李梓盟 张柯达 王晖 吴金虎 

机构地区：[1]湖北中医药大学药学院,武汉430065 [2]武汉市第三医院药学部,武汉430060

出　　处：《医药导报》2016年第6期632-636,共5页Herald of Medicine

基　　金：武汉市科技局学科带头人计划项目(201150530155);湖北省自然科学基金资助项目(2014CFC1045)

摘　　要：目的优选复方前愈水煎液中纯化多糖最佳工艺条件。方法采用水提醇沉、复溶、Sevag法除蛋白、透析法进行纯化,以苯酚-硫酸分光光度法测定多糖纯度,在单因素实验基础上,通过正交实验考察复溶固液比、除蛋白次数、透析时间等条件对复方前愈多糖纯度的影响。结果复方前愈多糖最佳纯化工艺条件为:固液比为1∶40(g/m L,W/V)、脱蛋白10次、透析18 h,在此条件下纯化多糖纯度可达69.04%,转移率51.84%。结论优选的纯化工艺流程简便易行,可用于复方前愈水煎剂中多糖的纯化。Objective To optimize the technological conditions of polysaccharide purification from compound Qianyu water decoction. Methods Water extraction and alcohol precipitation,resolution,Sevag method and dialysis method were used to purify polysaccharide.The purity of polysaccharide was measured with the phenol-sulfuric acid spectrophotometry. On the basis of single factor test,effects of redissolved solid-liquid ratio,number of protein removal,and dialytic time on polysaccharide purity of Qianyu were investigated by orthogonal test. Results The best conditions for purification of polysaccharide in Qianyu were as follows： liquid-solid ratio was 1 ∶ 40（ g / m L,W / V）,remove protein for 10 times,and dialysis for 18 h. The content of polysaccharide could reach 69.04%,and the transfer rate was 51. 84%. Conclusion The optimized purification process was simple and accurate.It can be used for polysaccharide purification in compound Qianyu water decoction.

关 键 词：前愈水煎液 复方 多糖 Sevag法 分光光度法 苯酚-硫酸 

分 类 号：R284.2[医药卫生—中药学]