一测多评法测定菊花中3个指标成分含量  

Simultaneous Ditermination of Three Indicators in Chrysanthemi Flos by Quantitative Analysis of Multi-Components by Single Marker

作　　者：黄渭[1] 王奕[1] 

机构地区：[1]武汉市中心医院药学部,武汉430014

出　　处：《医药导报》2016年第6期645-649,共5页Herald of Medicine

摘　　要：目的建立菊花中绿原酸、木犀草苷和3,5-O-二咖啡酰基奎宁酸的一测多评含量测定方法。方法采用高效液相色谱(HPLC)法,以绿原酸为内标物,建立其与木犀草苷、3,5-O-二咖啡酰基奎宁酸的相对校正因子,并进行含量测定,实现一测多评。同时采用外标法测定12批菊花中绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸含量验证一测多评法的准确性。结果采用外标法和一测多评法测得的木犀草苷和3,5-O-二咖啡酰基奎宁酸含量的平均相对误差分别为2.75%,1.33%。结论建立的校正因子重现性良好,在对照品缺乏的情况下,以绿原酸为内参物建立菊花中3种指标成分含量测定的一测多评法可行。Objective To establish a method for determination of chlorogenic acid,galuteolin and 3,5-O-dicaffeoyl quinic acid in chrysanthemums by quantitative analysis of multi-components by single marker（ QAMS）. Methods Using chlorogenic acid as the internal standard substance,the relative correction factors（ RCFs） of galuteolin and 3,5-O-dicaffeoyl quinic acid were determined by HPLC.The contents of chlorogenic acid,galuteolin and 3,5-dicaffeoyl quinic acid in 12 samples of chrysanthemums were authentically determined by the external standard method,so as to to verify the accuracy of the QAMS method. Results The average relative error of contents of galuteolin and 3,5-O-dicaffeoyl quinic acid was 2.75% and 1.33%by the external standard method and QAMS,respectively. Conclusion The established RCFs have good reproducibility.In case of lack of standard substances,the QAMS method with chlorogenic acid as an internal standard for determination of 3 indicators in chrysanthemum is feasible.

关 键 词：菊花 一测多评法 校正因子 相对 绿原酸 色谱法 高效液相 

分 类 号：R282.71[医药卫生—中药学]