基于体外药效学对土鳖虫不同酶解部位的抗凝活力研究  

Anticoagulant Activity of Different Enzymatic Hydrolysis Parts of Eupolyphaga Based on Pharmacodynamics in vitro

作　　者：田晨颖 窦家聪 刘国飞 高鹏[1] 柏道鸣 代龙[1] 

机构地区：[1]山东中医药大学,山东济南250355 [2]安徽生物肽产业研究院有限公司,安徽合肥230000

出　　处：《辽宁中医杂志》2016年第6期1299-1302,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金："十二五"国家自然科学基金资助项目(2013ZX09103002-021)

摘　　要：目的:通过对土鳖虫不同酶解产物进行体外抗凝药效学实验,筛选强活性部位。方法:以土鳖虫不同酶解产物的得膏率、总肽含量、抗凝活力为指标,以冷冻干燥将不同酶解产物制成冻干粉,利用体外溶血块实验、血液黏度测定比较其抗凝活力。结果:仿生酶解法(胃蛋白酶+胰蛋白酶)产物的得膏率41.84%、总肽含量为26.22 mg·m L^(-1)、其抗凝活力为2.9 U·m L^(-1),其体外溶血率最高,血液黏度最低。结论:与单一酶进行酶解相比,仿生酶解法所得酶解产物具有更强的抗凝活力。Objective： To screen the active fraction by pharmacological experiment with anti- thrombogenicity on different enzymatic hydrolysates of Eupolyphaga. Methods： With the extraction rate,the total content of peptide and anticoagulant activity as indexes,different enzymatic hydrolysates were made to be lyophilized powder by freeze- drying. Comparisons of anticoagulant activities were made in vitro by hemolysis test and blood viscosity measurement. Results： The yield from bionic enzymatic method（ pepsin ＋ trypsin） was 41. 84% and the total peptide content was 26. 22 mg·m L-1and the anticoagulant activity 2. 9 U·m L-1and this results had the highest hemolysis rate in vitro and the lowest blood viscosity. Conclusion： Compared with the single enzyme digestion,the yield from bionic enzymatic method has greater anticoagulant activity.

关 键 词：抗凝活力 体外药效学 冷冻干燥 酶解部位 土鳖虫 

分 类 号：R282.74[医药卫生—中药学]