黄芪预处理提高小鼠对肠缺血时间的耐受能力  

Tolerance for intestinal ischemia of mouse by astragalus preconditioning

作　　者：罗刚[1] 健陈 曦姚 伟锋 陈潮 金吴珊 

机构地区：[1]中山大学附属第三医院麻醉科,广州市510630

出　　处：《实用医学杂志》2016年第11期1760-1763,共4页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2014A020212707)

摘　　要：目的:研究黄芪(AS)预处理提高小鼠对小肠缺血时间耐受的作用。方法:C57BL/6小鼠随机分为假手术组(S组)、肠缺血再灌注组(IR组)、药物预处理组(ASIR组)。IR组和ASIR组均包含2个亚组,分别阻断肠系膜上动脉45 min(IRI组、ASIR1组)及60 min(IR2组、ASIR2组)后再灌注2 h。回肠末端组织行HE染色后光镜观察;ELISA检测血清内毒素(LPS)和二胺氧化酶(DAO)及肠黏膜肿瘤坏死因子α(TNF-α)含量;免疫荧光检测细胞色素C(Cyto C)的表达。结果:AS预处理显著降低肠黏膜Chiu′s评分;下调Cyto C的表达(P<0.05),减少血清LPS、DAO及肠黏膜TNF-α的含量;各损伤指标在ASIR2组较ASIR1组明显加重,AS预处理后两组均显著改善且上述指标比较差异无显著性。结论:AS预处理对缺血再灌注后肠黏膜具有明显的保护作用,使其对缺血时间的耐受增强。Objective To investigate the protective effects of astragalus preeonditioning on the tolerance of isehemia time of mouse small intestine. Methods C57BL/6 mice were randomly divided into 5 groups （n = 7）： sham operation group （Sham group）,intestinal isehemia repeffusion group （IR group） and astragalus preconditioning group （ASIR group）. IR group and ASIR group include 2 sub-groups respeetively, specifically, 2 h repeffusion was performed 45 min （ASIRI） and 60 min （ASIRI） after blocking superior mesenterie artery. Intestinal terminal morphology was observed by light microscope after HE coloration. Serum levels of LPS, DAO and intestinal mueosa TNF-α were measured by ELISA. Intestinal Cyto C expression were detected by immunofluorescence. Results Astragalus preconditioning reduces Chiu＇s score significantly. Expression of Cyto C was significantly down-regulated in astragalus preconditioning groups, and levels of LPS, DAO and TNF-α significantly decreased. The damages in IR2 group is obviously severe than in IR1 , but there were no significant differences between this two groups after pretreatment with astragalus. Conclusion Astragalus preeonditioning has obvious protective effects to intestinal ischemia reperfusion, and enhances the tolerance to longer time of ischemia.

关 键 词：黄芪 肠缺血再灌注 肠黏膜屏障 二胺氧化酶 

分 类 号：R285.5[医药卫生—中药学]