蛭丹化瘀组方对内毒素诱导肺损伤大鼠CGRP、ET-1的影响  

Effects of Zhi Dan Hua Yu prescription on CGRP and ET-1 of lung injury rats induced by LPS

作　　者：李朝霞[1] 欧阳俊摇 王雅丽[1] 赵晖[1] 沈素[2] 王丽丽[2] 李任[2] 

机构地区：[1]首都医科大学中医药学院,中医络病研究北京市重点实验室,北京100069 [2]首都医科大学附属北京友谊医院,北京100050

出　　处：《世界中医药》2016年第5期865-867,共3页World Chinese Medicine

基　　金：北京市科学技术委员会“十病十药”研发项目(编号:Z141100002214019)--“蛭丹化瘀组方用于治疗小儿病毒性肺炎的成药性研究”

摘　　要：目的:探讨蛭丹化瘀组方对内毒素诱导肺损伤大鼠血浆降钙素基因相关肽(CGRP)、内皮素-1(ET-1)、肿瘤坏死因子(TNF-α)的影响。方法:SD大鼠84只,雌雄各半,随机分为对照组、模型组、蛭丹化瘀组方3个剂量(33、16.5、8.25 g/kg)。大鼠气管内滴注LPS复制急性肺损伤大鼠模型,称重法检测大鼠肺含水量,放免法检测各组大鼠血浆ET、CGRP、TNF-α的变化。结果:模型组大鼠肺含水量较正常组明显升高(P<0.01),蛭丹化瘀组方(33 g/kg、16.5 g/kg)(P<0.01、P<0.05)以及阳性药地塞米松(P<0.01)可降低内毒素诱导肺损伤大鼠的肺含水量;与正常组比较,模型组大鼠血浆中内皮素(ET)、降钙素基因肽(CGRP)明显增高(P<0.01);蛭丹化瘀组方(16.5 g/kg、8.25 g/kg)和阳性药地塞米松可明显降低大鼠血浆ET含量,差异较模型组明显(P<0.01);蛭丹化瘀组方33 g/kg可明显增加血浆CGRP含量;与正常组比较,模型组大鼠血浆肿瘤坏死因子(TNF-α)明显增高(P<0.01);阳性药地塞米松及蛭丹化瘀组方(33 g/kg、16.5 g/kg、8.25 g/kg)均可明显降低大鼠血浆TNF-α含量(P<0.01)。结论:蛭丹化瘀组方方通过升高内毒素诱导肺损伤大鼠CGRP的含量,降低ET-1及TNF-α含量发挥对肺损伤大鼠的保护作用。Abstract Objective：To observe the effect of Zhi Dan Hua Yu prescriptionon CGRP,ET-1 and TNF-aof lung injury rats reduced byendotoxin. Methods ： A total of 84 SD rats withhalf males and half females were divided into control group, model group, Zhi Dan Hua Yu prescription group（33 g/kg, 16. 5 g/kg,8.25 g/kg）and dexamethasone group by random, detecting the changes ofwater- content inlung injury rats＇ lung;utilizing radioimmunoassay to detecting changes of ET and CGRP in rat plasma. Results：Compared to control group,lung water content in model group rats were significantly increased （P 〈 0. 01 ） , Zhi Dan Hua Yu prescription （33 g/kg, 16. 5 g/kg）and dexamethasone obviously reduced water content in lunginjmy rats. The endothelin （ET）and ealcitonin gene peptide（CGRP） in rat plasmaof model group were significantly increased （P 〈 0. 01 ）compared to that of control group; dexametha- sone reduced ET content（ P 〈 0. 01 ）and Zhi Dan Hua Yu prescription（ 16.5 g/kg,8.25 g/kg）reduced ET contant apparently（P 〈 0. 01 ） ; CGRP eontant in rat plasma of Zhi Dan Hua Yuprescription（ 33 g/kg）were increased significantly （P 〈 0. 01 ） ; The con- tent of tumor necrosis faetor-a（TNF-ct） was increased apparently（P 〈 0. 01 ） in comparison with that of control group ; dexametha- sone and Zhi Dan Hua Yu prescription（33 g/kg, 16.5 g/kg,8.25 g/kg）reduced TNF-ain rat plasma obviously（ P 〈 0. 01 ）. Con- clusion：Zhi Dan Hua Yu prescriptionprotect endotoxin-induced lung injury rats by increase CGRP content and reduce ET-1 and TNF-aeontent in rat plasma.

关 键 词：蛭丹化瘀组方 肺损伤 降钙素基因相关肽 内皮素-1 

分 类 号：R285.5[医药卫生—中药学]