高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定小儿清热宁颗粒中胆酸的含量  

Determination of Cholic Acid in Xiaoer Qingrening Granule by HPLC-ELSD

作　　者：崔梦丽 刘雅敏[1] 胡春月[1] 崔浩 陈庆[1] 苏梦[1] 朱宝玉[1] 

机构地区：[1]河南中医学院,郑州450046 [2]辅仁药业集团有限公司,郑州450008

出　　处：《世界中医药》2016年第5期903-905,909,共4页World Chinese Medicine

基　　金：国家科技重大专项(编号:2013ZX09201023)

摘　　要：目的：建立小儿清热宁颗粒中胆酸的含量测定方法;方法：样品制备采用甲醇超声30 min提取,色谱条件为色谱柱Venusil XBP C18（L）柱（4.6 mm×250 mm,5μm）,流动相甲醇-1%冰醋酸溶液（75∶25,v/v）,流速1.0 m L/min,柱温为25℃;检测器漂移管温度控制为40℃,载气气体流速为2.0 L/min,增益为4。结果：胆酸的工作曲线为lg A=1.582 lg C＋2.972 9（r=0.999 4）,进样量在0.502-2.51μg范围内呈良好的线性关系,回收率的RSD为1.969 6%。阴性对照无干扰。结论：所建立的含量测定方法专属性强,可用于小儿清热宁颗粒的质量控制。Abstract Objective：To establish an HPLC-ELSD method for determination of cholic acid in Xiaoer Qingrening Granule. Meth- ods：Samples were prepared by ultrasonic extraction of methanol for 30 min. Venusil XBP C18 （L） （4.6 mm x 250 mm, 5 μm） column was used with methanol -0.1% phosphoric acid solution （75： 25, v/v） with the mobile phase at 1.0 mL/min. The tem- perature of column was set at 25 ℃and drift tube was 40 ℃, The flow rate of gas was maintained at 2.0 L/min. Results ： The lin- earity was obtainedfrom 0. 502 -2.51 μg （r =0. 999 4）. The average recovery was 100. 532 1% with RSD = 1. 969 6%. Conclu- sion： The method is specific, and can be used for quality control of Xiaoer 0ingrening Granule.

关 键 词：高效液相色谱-蒸发光散射法 小儿清热宁颗粒 胆酸 

分 类 号：R284.1[医药卫生—中药学]