疯草解毒复方中药制剂的药代动力学研究  

Study on Pharmacokinetics of Compound Traditional Chinese Medicine Preparation for Locoweed Detoxication

作　　者：郝宝成[1] 权晓弟 叶永丽[2] 高旭东[2] 黄鑫[1] 王学红[1] 刘建枝[3] 王保海[3] 梁剑平[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省新兽药工程重点实验室,兰州730050 [2]西北民族大学生命科学与工程学院,兰州730030 [3]西藏自治区农牧科学院畜牧兽医研究所,拉萨850000

出　　处：《中国畜牧兽医》2016年第6期1550-1556,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家高新技术研究发展计划(863计划)(2011AA10A214);西藏横向委托项目(2011-2014);中国农业科学院科技创新工程兽用天然药物创新工程(CAAS-ASTIP-2014-LZHPS-04)

摘　　要：本试验旨在探讨疯草解毒用复方中药制剂在大鼠体内的药代动力学特性。在确定制剂中有效成分的基础上,采用紫外分光光度法检测大鼠经口服药物制剂后体内有效成分的吸收情况,并计算相应的药代动力学参数。本试验建立了适用于检测8种成分的紫外光谱分析法;制剂经口服给药后能在胃肠道被快速吸收,1h左右大鼠体内各组分的血药浓度达到最大,药效发挥迅速;党参皂甙、多糖和阿魏酸代谢速度快,甘草酸、柴胡皂苷、黄芪甲苷、升麻素苷的代谢产物在体内作用时间较长。复方中药制剂在试验大鼠体内的药代动力学过程基本符合二室模型(陈皮苷除外)。所选用的测定方法简便、准确度和稳定性较高,适用于该制剂的药代动力学研究。The test was conducted to study the pharmacokinetics of compound traditional Chinese medicine preparation for locoweed detoxication in rats.After oral drug preparations,the absorption circumstances of active ingredients in vivo were detected by ultraviolet spectrophotometry and then the relative pharmacokinetic parameters were calculated based on identifying the effective components.The results showed that a suitable ultraviolet spectrum analytical method was established for 8compounds at first.It had shown that the effective components were absorbed into the blood quickly after oral administration,and the maximum plasma concentration of each composition was got after about 1h,indicating that the efficacy developed rapidly.The metabolic rates of Codonopsis pilosula saponin,polysaccharide and ferulic acid were fast,while the action time of the metabolites of glycyrrhizic acid,saikosaponin,astraloside and prim-O-glucosylc-imifugin were longer.The results suggested that pharmacolinetic process of this medicine preparationin rats basically conformed with two compartment model（expect the hesperidin）.The determination method was simple,and had high accuracy and stability,as well as suitable for the pharmacokinetic study.

关 键 词：复方中药制剂 药代动力学 紫外分光光度法 疯草 

分 类 号：S859.7[农业科学—临床兽医学]