福建地方鸡B亚群禽白血病病毒的分离鉴定  

Isolation and identification of a subgroup B avian leukosis virus from local chicken breeds in Fujian

作　　者：赵金荣[1] 曾宇坤 朱二鹏 周五朵 吴异健[1] 胡万荣[1] 吴宝成[1] 吴晓平[1] 

机构地区：[1]福建农林大学动物科学学院,福建福州350002

出　　处：《中国兽医科学》2016年第6期769-774,共6页Chinese Veterinary Science

基　　金：国家自然科学基金青年项目(31402227);福建农林大学青年基金项目(2011xjj10)

摘　　要：在对福建某地方鸡群进行禽白血病流行病学调查的基础上,对疑似病例进行p27抗原检测,取阳性血清接种DF-1细胞分离病毒。采用PCR及间接免疫荧光试验鉴定其亚群并对其gp85基因进行序列分析。结果显示,分离株FJ15HT0的B亚群特异性PCR检测呈阳性;IFA显示感染该毒株的DF1细胞中可检测到p27抗原,而未检测到J亚群特异性囊膜蛋白的表达;对该毒株gp85序列进行分析,发现与多株ALV-B参考毒株氨基酸序列的同源性为91.6%～98.8%,其中与GX10LS01株同源性最高为98.8%,与A、C、D、E亚群同源性在69.4%～84.2%,与J亚群同源性仅为36%～38%。该研究首次分离并鉴定河田鸡群中ALV-B,为后续的研究奠定了基础。Based on the epidemiological investigation of Avian leukemia（AL） in native breed in Fujian Province,the suspected cases were chosen for p27 antigen test of Avian leukosis virus（ALV）, and DF-1 cells were infected with the positive serum for viral isolation.The subgroup of isolated ALV-FJ15HT0 strain was identified using PCR technique and indirect immunofluorescence assay（IFA）, and then gp85 gene was cloned for sequence analysis.The results showed that PCR amplification with ALV-B-specific primers was positive;p27 antigen was detected in FJ15HT0 infected DF-1 cells under fluorescent microscope, while ALV-J-specific envelope proteins were undetected;gp85 gene deduced amino acid sequence analysis showed that the identity of FJ15HT0 strain and other ALV-B reference strains ranged from 91.6% to 98.8%,among which the highest identity（98.8%）was with GX10LS01 strain,when compared with ALV-A,C,D and E,the amino acid identity ranged from 69.4%to 84.2%,in addition,the gp85 identity between FJ15HT0 strain and ALV-J was only 36%-38%.This study would provide theoretical foundations for further ALV-associated studies on native breed.

关 键 词：地方鸡群 B亚群ALV 分离鉴定 

分 类 号：S852.659.6[农业科学—基础兽医学]