纤维蛋白粘合剂应用于犬小肠端端吻合术的临床组织学观察  

Clinical histological observation of canine intestine end using fibrin glue for recovery of the end with anastomosis

作　　者：韩鹏[1] 陈智华[1] 郭慧琳[2] 范希萍[1] 高宇航[1] 范彩彩[1] 年聚盆 亢心 赵苗苗[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]甘肃省动物疫病预防控制中心,甘肃兰州730046

出　　处：《中国兽医科学》2016年第6期793-798,共6页Chinese Veterinary Science

基　　金：甘肃省农牧厅生物技术专项(GSSW-2016-26)

摘　　要：本试验将24只犬随机分为两组,进行小肠端端吻合术。试验组采用纤维蛋白粘合剂配合缝线简易缝合的方法进行吻合,对照组仅采用缝线的方法进行吻合。通过记录肠管吻合术时间、观察术后吻合肠管大体形态、测定术后第14、28天吻合口爆破压及术后第28天吻合口肠管内径比率,对术后第3、7、14、28天吻合口组织进行病理组织学观察,并测定术后第7、28天吻合口组织内Caspase-3的质量分数,以探讨纤维蛋白粘合剂对犬小肠端端吻合术愈合的影响。结果显示,肠管吻合术时间试验组显著短于对照组(P<0.05),试验组术后第3天仅有1只犬在肠管吻合部位发生了极少粘连,所有犬均未发生肠梗阻。对照组所有犬术后吻合部位均有不同程度的粘连,术后第28天有1只犬发生了肠梗阻。术后第28天,试验组吻合口内径比率大于对照组(P<0.05)。术后第14天和第28天,试验组吻合口爆破压均高于对照组,差异显著(P<0.05)。术后第7、28天Caspase-3的质量分数试验组均高于对照组(P<0.05)。术后第3、7、14、28天取吻合口组织进行病理组织学观察,发现愈合过程中试验组炎性反应较对照组轻,试验组成纤维细胞出现较早。上述结果表明,应用纤维蛋白粘合剂可缩短肠管吻合术时间,加快愈合进程。In this experiment,24 dogs were randomly divided into experimental group and control group for canine intestine end operation recovery from anastomosis.The experimental group was manipulated with simple suture and fibrin glue,while the control group was only routinely sutured.By recording the operation time,observing the general shape,testing anastomotic blasting pressure on day14 and 28 post-operation and anastomotic inner diameter ratio on day 28 post-operation,measuring the content of caspase-3 on day 7 and 28 post-operation,the influence of fibrin glue on canine intestine end operation recovery were explored.In result,operation time of the experimental group was shorter than that of in the control group（P〈0.05）.On day 3 post-operation,only one dog emerge adhesion in theexperimental group,all dogs did not occur intestinal obstruction.All the dogs in the control group had different degrees of adhesions,and one dog happened obstruction on day 28 post-operation.The anastomotic inner diameter ratio of the experimental group is hihger than that in the control group（P〈0.05）on day 28 post-operation.On day 14 and 28 post-operation,the anastomotic blasting pressure of the experimental group was higher than that of in the control group（P〈0.05）.On day 7 and 28 post-operation,the content of caspase-3 in the experimental group is hihger than that in the control group（P〈0.05）.Anastomotic tissues were took for histopathological observation,and it was found that the inflammatory in healing process in the experimental group was lighter than that of in the control group,and the experimental group appeared fibroblasts earlier.The above-mentioned results showed that the fibrin glue shortens the recovery time post-operation and speeds up process of healing.

关 键 词：纤维蛋白粘合剂 小肠端端吻合术 成纤维细胞 胶原纤维 CASPASE-3 

分 类 号：S857.12[农业科学—临床兽医学]