聚焦微波助脱除纤维素提取杜仲籽壳中杜仲胶  

Focused Microwave-Assisted Removal of Cellulose Coupled with Solvent Extraction of Gutta-percha from Eucommia ulmodies Seeds Shell

作　　者：张月[1] 王素素[1] 李辉[1,2] 向承启 

机构地区：[1]吉首大学化学化工学院 [2]吉首大学植物资源保护与利用湖南省高校重点实验室,吉首416000

出　　处：《天然产物研究与开发》2016年第6期904-909,942,共7页Natural Product Research and Development

基　　金：国家自然科学基金面上项目(21077042);湖南省高校科技创新团队支持计划

摘　　要：本研究采用聚焦微波提取法先脱除杜仲籽壳中的纤维素,再用溶剂提取杜仲胶。用实验因子设计优化了聚焦微波提取条件,从三个水平考察了四个因子,即微波辐照时间、碱液浓度、溶剂用量及微波功率对纤维素脱除效率的影响。脱除纤维素后的杜仲材料用石油醚为溶剂提取杜仲胶。结果表明:聚焦微波辐照脱除杜仲籽壳纤维素的优化条件为:辐照时间:20 min;氢氧化钠浓度:12.5%;溶剂用量:17.5 m L/g;微波功率:70%。与常规法碱提法提取杜仲胶相比,聚焦微波助提法提取率高,单次提取率达64.18%,提取时间短,一次提取需时小于3 h,而常规法约需30 h。两种方法所获产品纯度相差不大,显示了聚焦微波助提法提取杜仲胶的高效能。In the present study, a focused microwave-assisted solvent extraction method was developed for the removal of cellulose in Eucommia ulmodies seeds shell ,followed by solvent extraction of gutta-percha. A L9 （34） orthogonal factorial design for the optimization of removal conditions was used to evaluate the influence of four parameters ,namely irradiation time, NaOH concentration, solvent volume and microwave power, on removal efficiency of cellulose. Then, the material obtained after removing cellulose was utilized to extract gutta-percha. Results indicated that the optimized conditions for the removal of cellulose were as follows：irradiation time of 20 min, 12.5 % NaOH solution as extraction solvent, 17.5 mL solvent per gram material and 70% microwave power. Compared with the conventional NaOH extraction method, the pro- posed method possessed higher extraction efficiency for gutta-percha, with a value of 64.18% for a single circle, in a much less time （ less than 3.0 h for the proposed method and about 30 h for the conventional one）, with a comparable purity of gutta-percha. These results implied that the focused microwave-assisted extraction method was a promising method for the extraction of gutta-percha.

关 键 词：杜仲胶 微波助提取 杜仲籽 溶剂提取 

分 类 号：Q946.91[生物学—植物学] P284.2[天文地球—地图制图学与地理信息工程]