桃色顶孢霉代谢产物对绿豆白粉病的防治作用  

Biological Control on Powdery Mildew of Mungbean by Acremonium persicinum Fermentation

作　　者：林志伟[1] 张新[1] 肖亚静[1] 左豫虎[1] 于立河[1] 孙冬梅[1] 

机构地区：[1]黑龙江八一农垦大学,大庆163319

出　　处：《天然产物研究与开发》2016年第6期934-937,859,共5页Natural Product Research and Development

基　　金：国家科技支撑计划(2014BAD07B05-06)

摘　　要：应用桃色顶孢霉发酵液中蛋白粗提物、三唑酮处理感病绿豆叶片和植株,比较分析病斑变化和综合病情指数,并测定不同处理植株新生叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量,探究桃色顶孢霉发酵液粗提蛋白对绿豆白粉病的影响。结果表明,喷施粗提蛋白的感病植株叶片的病斑变化明显,且新生叶片鲜绿,无白粉病菌侵染症状。与喷施无菌水的对照相比,喷施粗提蛋白的感白粉病绿豆植株叶片CAT酶活性提高了71.70%,差异显著,而POD酶活性降低了8.45%,且叶片MDA含量降低了29.81%。试验结果证实了桃色顶孢霉发酵液的蛋白粗提物对绿豆白粉病具有一定的防治效果,并能影响到植物相关抗逆酶的活性。In order to study the relationship between the crude protein extract of fermentation of Acremonium persicinum and powdery mildew on mungbean,the disease index was calculated, and the activity of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT）, the content of malohdialdehyde （MDA） were all determined. The results showed that the disease index of leaves spraying by crude protein extract was the lightest and new leaves were no longer infected by powdery mildew. Compared with the treatment of spraying sterile water, CAT activity increased by 71.70% （ P 〈 0.05 ） in the treatment of spraying crude protein, but POD activity decreased by 8.45% （ P 〈 0.05 ）, MDA content decreased by 29.81% （P 〈0.05 ）. The result confirmed a certain control effect of crude protein extracted from fermen- tation of A. persicinum on mungbean powdery mildew and on relative anti-resistance enzyme activities of mungbean.

关 键 词：桃色顶孢霉 发酵液粗提蛋白 绿豆白粉病 病情指数 抗逆酶 

分 类 号：Q939.95[生物学—微生物学]