锌指蛋白15在猪前体脂肪细胞分化中的作用  

Role of Zinc Finger Protein15 in Differentiation of Porcine Preadipocyte

作　　者：柴孟龙[1] 李青莹 姜昊[2] 刘红羽[1] 张嘉保[2] 吕文发[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]吉林大学动物科学学院,长春130062

出　　处：《吉林农业大学学报》2016年第3期325-329,335,共6页Journal of Jilin Agricultural University

基　　金：吉林省重点科技攻关项目(20140204077NY)

摘　　要：首先分离猪前体脂肪细胞进行体外培养和诱导分化,通过荧光定量PCR明确KLF15基因在猪前体脂肪细胞分化过程中mRNA的表达规律。随后利用脂质体2000将KLF15-siRNA转染至前体脂肪细胞并诱导分化,待前体脂肪细胞分化为成熟脂肪细胞后分别利用荧光定量PCR、油红O染色等方法明确脂肪细胞分化标志基因PPARγ、C/EBPα、AP2的表达变化及脂肪细胞甘油三酯沉积能力。结果表明:KLF15基因mRNA表达量在猪脂肪细胞分化过程中随时间而变化,呈现出先上升后下降的趋势,在分化24 h时mRNA表达量最高。与对照组相比,KLF15基因表达被抑制后,PPARγ、C/EBPα、AP2表达量显著降低,甘油三酯含量显著降低,脂滴明显减少、变小。研究结果提示,KLF15参与了调控前体脂肪细胞分化的过程,可影响脂肪细胞中甘油三酯的合成及脂质的沉积。This study selected subcutaneous fat of Junmu-1 piglets for in vitro culture, induction of differentiation, and identification experiments. The mRNA expression levels of KLF15 were detected through fluorescence quantitative PCR. Cells were treated with KLF15-siRNA and subjected to in- duction of differentiation. The changes in oil droplets were observed using oil red O staining. The changes in the mRNA levels of the preadipocyte differentiation genes PPARγ, C/EBPα, and AP2 were detected using fluorescence quantitative PCR. The results showed that the mRNA expression levels of the KLF15 gene changed over time during the differentiation process of porcine adipocytes. The mRNA expression level was the highest at 24 h of differentiation. After the mRNA expression level of KLF15 was inhibited, the level of triglycerides significantly decreased （P〈0. 05） lipid droplets significantly decreased in number and became smaller; and the expression levels of PPARγ, C/EBPα and AP2 significantly decreased （P〈 0. 05 ）. The above results indicated that KLF15 can participate in the regulation of preadipocyte differentiation, and affect triglyceride synthesis in adipocytes as well as lipid deposition.

关 键 词：猪 KLF15 前体脂肪细胞 分化 脂质沉积 

分 类 号：S828.2[农业科学—畜牧学]