Blimp-1和Bcl-6在不明原因复发性流产患者蜕膜组织中表达增强  

Increased expressions of Blimp-1 and Bcl-6 in the deciduas of patients with unexplained recurrent spontaneous abortion

作　　者：龚巧巧[1,2] 朱玥洁[1] 庞盼 黄玉红[2] 赵骏达 赵静[1] 腊晓琳[1] 丁剑冰[2] 

机构地区：[1]新疆医科大学第一附属医院 [2]新疆医科大学基础医学院免疫学教研室,新疆乌鲁木齐830011

出　　处：《细胞与分子免疫学杂志》2016年第7期963-967,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81160200);新疆自治区自然科学基金(2013211A087);新疆自治区高校学科建设(XYDXK50780328)

摘　　要：目的通过检测B淋巴细胞诱导成熟蛋白1(Blimp-1)和B细胞淋巴瘤分子6(Bcl-6)在不明原因复发性流产(URSA)患者蜕膜组织中的表达水平,探讨两因子与URSA的关系。方法收集URSA患者(URSA组)和正常怀孕者(对照组)的蜕膜组织,采用实时定量PCR法检测蜕膜组织Blimp-1和Bcl-6的mRNA水平,免疫组织化学技术检测蜕膜组织Blimp-1和Bcl-6的蛋白水平,采用Pearson相关分析Blimp-1和Bcl-6的相关性。结果与对照组相比,URSA组蜕膜组织中Blimp-1和Bcl-6的mRNA均增加,Blimp-1的蛋白水平也显著增加,而Bcl-6蛋白表达水平增加不明显。Blimp-1和Bcl-6的mRNA表达水平显著正相关。结论 URSA组患者蜕膜组织中Blimp-1和Bcl-6表达增强。Objective To oberseve the expressions of B cell lymphoma 6 （Bcl-6） and B lymphocyte-induced maturation p（otein 1 （ Blimp-1 ） in the decidua of patients with unexplained recurrent spontaneous abortion （ URSA）, and investigate the roles that Bcl-6 and Blimp-1 act in URSA. Methods Decidual tissues were collected from patients with URSA （ URSA group） and normal pregnant women （control group）. Then, we detected the expressions of Bcl-6 and Blimp-1 in the deciduas of the two groups using real-time fluorescent quantitative PCR and immunohistochemistry. The relationship between Blimp-1 and Bcl-6 was estimated by Pearson＇s correlation analysis. Results Compared with the control group, the levels of Blimp-1 mRNA and protein, Bcl-6 mRNA significantly increased in the URSA group. However, Bcl-6 protein was raised insignificantly in URSA group. Furthermore, there was a positive correlation between Bcl-6 and Blimp-1 at the level of mRNA. Conclusion The expressions of Bcl-6 and Blimp-1 are enhanced in the deciduas of URSA patients.

关 键 词：不明原因复发性流产(URSA) 滤泡辅助型T淋巴细胞 B淋巴细胞诱导成熟蛋白1(Blimp-1) B细胞淋巴瘤分子6(Bcl-6) 

分 类 号：R392.12[医药卫生—免疫学] R714.21[医药卫生—基础医学]