河南口岸截获鼠类DNA条形码鉴定  

Identification of intercepted Rattus nitidus by DNA barcoding at Henan port

作　　者：张勤 王晓梦 岳巧云 高洁 邱德义 

机构地区：[1]河南出入境检验检疫局技术中心医学媒介生物实验室,郑州450003 [2]中山出入境检验检疫局,广东中山528403

出　　处：《中国媒介生物学及控制杂志》2016年第3期280-282,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的利用DNA条形码技术对河南机场口岸截获的鼠类样本进行鉴定。方法提取该鼠类样本基因组DNA,以通用引物Bat L5310和R6036R进行细胞色素C氧化酶亚基Ⅰ(COⅠ)基因片段的扩增并测序,将测序结果与Gen Bank及BOLD Systems v3中的序列进行比对,应用Mega 4.1软件构建系统发育树。结果该鼠样本COⅠ基因扩增片段去除引物后为702 bp,序列比对结果及系统进化关系表明其与大足鼠的同源性为99.43%,该鼠样本为大足鼠。结论 DNA条形码技术在鼠类鉴定中可弥补形态学鉴定的缺陷,是一种简单有效的鼠类鉴定方法。Objective DNA barcoding based on CO I gene was used to identify an unknown rat species captured at Henan port. Methods Genomic DNA of the unknown rat species was extracted, the international primer BatL5310 and R6036R were used to amplify CO I gene. Purified PCR product was sequenced and blasted in the GenBank and BOLD Systems v3. Phylogenetic trees were built with Mega 4.1. Results The amplified CO Ⅰ fragment, without primer, of the unknown rat species was 750 bp in size and the sequence was 99.43% identical to the published sequence of Rattus nitidus. The unknown rat species was identified as R. nitidus. Conclusion DNA barcoding is a simple and effective method for the identification of rat species and can be the alternative method of morphological identification.

关 键 词：DNA条形码 分子鉴定 大足鼠 

分 类 号：S443[农业科学—农业昆虫与害虫防治]