TQ纯天然植物蚊虫驱避剂质量控制及药效学研究  

Study on the quality control and repellent efficacy of TQ natural plant mosquito repellent

作　　者：沈睿娟 毛羽[1] 谭丰[1] 杨邦祝 李艳[1] 徐作军[1] 舒莎[1] 

机构地区：[1]西南民族大学,四川成都610041

出　　处：《中国媒介生物学及控制杂志》2016年第3期283-287,共5页Chinese Journal of Vector Biology and Control

摘　　要：目的建立高效无毒TQ纯天然植物蚊虫驱避剂的质量控制标准,研究TQ纯天然植物蚊虫驱避剂的驱蚊效果。方法选用川芎、姜黄、羌活、蛇床子、桉叶、薄荷6种药材的挥发油复方制备TQ纯天然植物蚊虫驱避剂,采用薄层色谱法对制剂中的6种成分进行定性鉴别;采用高效液相色谱法测定制剂中的藁本内酯含量;采用气相色谱法测定制剂中的薄荷脑含量;根据国标研究TQ纯天然植物蚊虫驱避剂的驱蚊作用。结果薄层色谱法可鉴别6种成分,斑点清晰,分离度好;高效液相色谱法测定藁本内酯,在1.08-6.48μg范围呈现良好的线性关系（r=0.999 6）,平均回收率为99.12%,TQ蚊虫驱避剂含藁本内酯≥19.80 mg/ml;气相色谱法测定薄荷脑,在0.175-0.875μg范围呈现良好的线性关系（r=0.999）,平均回收率为99.95%,TQ蚊虫驱避剂含薄荷脑不得少于6.10 mg/ml;白纹伊蚊攻击力实验合格,TQ纯天然植物蚊虫驱避剂有效保护时间＆gt;6 h。结论制定的驱避剂质量标准操作简便易行、准确可靠、灵敏度高、重现性好,可作为TQ纯天然植物蚊虫驱避剂的质量控制方法。TQ纯天然植物蚊虫驱避剂有效保护时间〉6 h,达国家驱避剂登记的A级标准。Objective To establish the quality standard of TQ natural plant mosquito repellent, and study its efficacy. Methods Volatile oil of Chuanxiong, turmeric, Notopterygium, Cnidium, eucalyptus and peppermint were used to prepare TQ natural plant mosquito repellent. Volatile oil of Turmeric, Ligustieum chuanxiong Hort, peppermint, eucalyptus and Notopterygium in TQ natural plant mosquito repellent were identified by TLC. A HPLC method to detect figustilide was established. And the content of menthol was determined by GC. GB/T 13917.10-2009 are adopted to study TQ natural plant mosquito repellent＇ s effect. Results The TLC spots of Volatile oil from Turmeric, Ligustieum chuanxiong Hort, peppermint, eucalyptus and Notopterygium were clear with good separation. The linear ranges of ligustilide was 1.08-6.48 μg, the average recovery rate was 99.12%, content of Ligustilide must not be less than 19.80 mg/ml; The linear ranges of menthol was 0.175-0.875 μg, the average recovery rate was 99.95%, content of menthol must not be less than 6.10 mg/ml. Test standard was met by Aedes albopictus challenge test, TQ biological repellents protect longer than 6 h. Conclusion The quality standards are simple but accurate, which can be used to control the quality of TQ natural biological repellents. TQ natural plant mosquito repellent sprays has obvious repellent effect, and can protect human from mosquitos for more than 6 h, reaching the A level of the national standard.

关 键 词：植物挥发油 驱避剂 蚊虫 藁本内酯 

分 类 号：S482.38[农业科学—农药学] R384.1[农业科学—植物保护]