人主动脉夹层与正常主动脉基因表达谱的差异  

Screening gene expression profiles of both aortic dissection and normal aorta by microarray

作　　者：张良[1] 于存涛[1] 常谦[1] 罗新锦[1] 丘俊涛 刘燊[1] 

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心阜外医院心血管疾病国家重点实验室,北京100037

出　　处：《临床心血管病杂志》2016年第6期580-585,共6页Journal of Clinical Cardiology

基　　金：国家自然科学基金项目(No:81270385)

摘　　要：目的:利用基因芯片技术研究人主动脉夹层组织与正常主动脉组织基因表达谱的差异。方法:选取主动脉夹层病例标本5例,正常主动脉标本4例。提取总RNA,并反转录成cDNA、体外转录合成aRNA后与芯片进行杂交,对结果进行分析。同时对表达谱筛选出MYLK、PKD1、MYH11、SOD3、FLNA、TAGLN 6个差异基因进行基因转录水平的定量验证。结果:人主动脉夹层组与正常主动脉组比较基因表达差异倍数大于2的基因共有1 661个,其中有997个基因上调表达,664个基因下调表达。6个基因RT-qPCR验证结果表明,与正常组相比,6个基因都下调表达,其中MYLK、PKD1、MYH11、SOD3、TAGLN基因下调表达是有显著性差异的(P≤0.05),FLNA基因没有显著性差异(P>0.05)。结论:通过全基因组表达谱芯片可以筛选主动脉夹层与正常主动脉表达差异基因,同时结合RT-qPCR验证,为研究主动脉夹层提供新的思路。Objective：To investigate the difference of gene expression profile in aortic dissection and normal aorta.Method：The total RNA of 5cases of aortic dissection and 4normal aorta were isolated and cDNA was synthesized,biotin were synthesized by in vitro transcription.The mixed probes were hybridized then aRNA labeled by with Affymetrix GeneChip？Human Genome U133 Plus 2.0Array.After scanning and image processing,the different gene expression profiling of aortic dissection and normal aorta was investigated. MYLK,PKD1,MYH11,SOD3,FLNA and TAGLN genes from profiles were validated by RT-qPCR.Result：Totally 1661 genes had more than two fold changed expression in aortic dissection,which included 997 upregulated and 664 downregulated genes.Different expressed genes were mainly involved in Cellular immune response,inflammatory reaction,cell signaling cascade amplification and so on.The results of RT-qPCR were not completely consistent with the results of gene microarray.Compared with the normal group,the 6genes were downregulated expression,MYLK,PKD1,myh11,SOD3,TAGLN were significant difference（P〈0.05）and FLNA gene was no significant difference（P〉0.05）.Conclusion：Gene differentially expressed in aortic dissection identified by microarray can provide the molecular basis for the study of the pathogenesis of aortic dissection.

关 键 词：基因表达谱 主动脉夹层 实时荧光定量PCR 

分 类 号：R543.1[医药卫生—心血管疾病]