补肾填精益髓法对慢性再生障碍性贫血患者血清TGF-β、IL-10及Foxp3 mRNA表达的影响  

The Effect of Bushen Tianjing Yishui(补肾填精益髓法,BTYD) on Serum TGF-β, IL-10 and Foxp3 mRNA in Patients with Chronic Aplastic Anemia(CAA)

作　　者：吴玉霞[1] 袁忠[1] 马西虎[1] 黄志惠[1] 费飞[1] 

机构地区：[1]新疆医科大学附属中医医院,新疆乌鲁木齐830011

出　　处：《中医药导报》2016年第13期39-42,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2010-2011年中医药行业科研专项(201107001);项目名称:慢性再生障碍性贫血致重因素中医干预方案的研究

摘　　要：目的:观察补肾益髓法对慢性再生障碍性贫血(CAA)患者血清TGF-β、IL-10及Foxp3 mRNA表达的影响。方法:56例CAA患者首先按照辨证分层,再随机分为治疗组与对照组,两组均予相同的西医治疗,在此基础上,治疗组予依据辨证分型的受试药物,对照组施以与受试药外观、气味一致的模拟剂。各组疗程均为6个月。比较两组临床疗效、T细胞亚群的变化及血清TGF-β,IL-10及Foxp3 mRNA表达水平变化。结果:(1)总有效率治疗组为82.1%,对照组为46.4%,差异有统计学意义(P<0.05)。(2)两组T细胞亚群变化比较:治疗后治疗组CD3、CD8、CD4表达与对照组比较,差异均有统计学意义(P<0.05),治疗组CD3、CD8细胞表达下降、CD4表达水平增高,优于对照组。(3)血清TGF-β、IL-10、Foxp3 mRNA比较:治疗后治疗组血清IL-10、TGF-β水平与对照组比较,差异均有统计学意义(P<0.05)。治疗组治疗后血清TGF-β下降,IL-10及Foxp3 mRNA上升,与治疗前比较,差异均有统计学意义(P<0.05),与对照组治疗后比较,差异有统计学意义(P<0.05);治疗后治疗组肾阳虚型血清IL-10水平高于其他证型,而TGF-β水平低于其他证型,差异有统计学意义(P<0.05)。结论:补肾填精益髓中药具有改善CAA患者免疫紊乱的作用。Objective： To observe the effect of Bushen Tianjing Yishui on serum TGF-β, IL-10 and Foxp3 mRNA in patients with Chronic aplastic anemia （CAA）. Methods： The 56 patients with CAA were randomly as- signed two groups. The 28 patients orally administered with BTYD in the treatment group and were divided into 3 Syndrome Types, and 28 administered with the same appearance and smell simulation agent in the control group. All patients received the usual western therapy. The therapeutic course was 6 months. T Cell Subset, serum TGF-~, IL-10 and Foxp3 mRNA were observed before and after treatment in two groups and the levels of serum TGF-β, IL-10 and Foxp3 mRNA in three TCM syndromes in treatment group after treatment was eval- uated. Results： （1） The total effective rate was 82.1% in the treatment group, and 46A% in the control group,re- spectively, showing significant difference between the two groups （P〈0.05）. （2） The result suggest that the change of T Cell Subset was significant difference between the two group （P〈0.05）; The declining levels of CD3 and CD8, and the rising levels of CD4 in the treated group were superior to those in the control group （P〈0.05）. （3） The result suggest that the levels of TGF-β decreased and IL-10, Foxp3 mRNA increased and there was signifi- cant difference between before and after treatment the treatment group and after treatment in two groups （P〈0.05）. The declining levels of TGF-β and The rising levels of IL-10 and Foxp3 mRNA were superior to the other syndromes group（P〈0.05）. Conclusion： Bushen Tianjing Yishui Decoction （BTYD） could optimizing immune response in CAA.

关 键 词：慢性再生障碍性贫血 补肾填精益髓法 血清TGF-β IL-10 FOXP3 mRNA 

分 类 号：R255.7[医药卫生—中医内科学]