GC法测定湿疹软膏中冰片和薄荷脑含量  

Determination of Borneol and Menthol in Eczema Ointment by GC

作　　者：李篮[1] 何群[2] 

机构地区：[1]湖南化工职业技术学院,湖南株洲412004 [2]湖南中医药大学,湖南长沙410208

出　　处：《中医药导报》2016年第13期97-100,103,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的：建立湿疹软膏中薄荷脑与冰片的含量测定方法。方法：采用气相色谱法,用乙酸乙酯提取薄荷脑、冰片,以水杨酸甲酯为内标物,使用PEG-20M色谱柱,用氢火焰离子检测器检测。结果：通过方法学考察,薄荷脑在0.05012~0.5012 mg/m L范围内呈良好的线性关系,平均回收率为99.60%（n=9）。冰片在0.06032~0.6032 mg/m L范围内呈良好的线性关系,平均加样回收率为99.24%（n=9）。结论：该方法简便、准确、重复性好、精密度高。可用于湿疹软膏的工艺研究。Objective： To establish a method to determine menthol and borneo1 in eczema ointment. Meth- ods： Menthol and borneol contents were determined by GC, extraction of menthol and borneol with ethyl acetate, Methylmethyl salicylate used as the internal standard, using the PEG-20M column, FID as detector. Results： A good linearity between the concentration and peak area value when the concentrations of menthol at 0.05012 - 0.50121 mg/mL. The average recovery of menthol is 99.60% （n=9）.A good linearity between the concentration and peak area value when the concentrations of borneol at 0.06032 -0.6032 mg/mL, and the average recovery of borneol is 99.24% （n=9）. Conclusion： The method is simple, accurate, reproducible and high precision; it can be used to control the quality of eczema ointment.

关 键 词：气相色谱 湿疹软膏 冰片 薄荷脑 

分 类 号：R284.1[医药卫生—中药学]