丁酸钠对特重度烧伤大鼠脏器功能及血流量的影响  

Effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury

作　　者：唐富波 张文华[3] 李雨梦[3] 李静远[3] 刘锐 胡森[3] 白晓东[1] 

机构地区：[1]武警总医院烧伤整形科,北京100039 [2]武警后勤学院,天津300309 [3]解放军总医院第一附属医院全军创伤修复与组织再生重点实验室暨皮肤损伤修复与组织再生北京市重点实验室,北京100048 [4]哈尔滨市第五医院烧伤整形科,150040

出　　处：《中华损伤与修复杂志（电子版）》2016年第2期90-95,共6页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：国家自然科学基金(81471872);国家发改委卫星示范专项[发改办高技(2013)2140号]

摘　　要：目的研究丁酸钠对特重度烧伤大鼠脏器功能及血流量的影响。方法雄性SD大鼠48只,体重(250±20)g,随机数字表法分为假伤组、烧伤组和丁酸钠组,每组16只。丁酸钠组与烧伤组采用沸水烫大鼠背部15 s、腹部8 s,造成50%TBSAⅢ度烧伤,伤后分别立即腹腔注射丁酸钠(400 mg/kg)与等体积0.9%氯化钠溶液。假伤组37℃温水浸泡后腹腔注射等体积0.9%氯化钠溶液。伤后3 h和6 h采用激光多普勒血流仪测定肝、肾、小肠黏膜血流量;腹主动脉取血检测血清谷丙转氨酶(ALT)、肌酐(Cr)、肌酸激酶同工酶(CK-MB)和二胺氧化酶(DAO);处死大鼠取脏器组织采用干湿重法检测心、肺、肝、肾和小肠含水率。结果丁酸钠组伤后3 h肾和小肠黏膜血流量[(93.56±11.56)、(84.65±12.64)BPU]分别高于烧伤组[(80.71±10.53)、(59.64±11.82)BPU],差异均有统计学意义(t=2.324、4.087,P值均小于0.05);心、肺、小肠含水率[(72.35±1.93)%、(69.56±1.83)%、(63.75±2.58)%]以及CK-MB水平(2794.56±291.54)U/L分别低于烧伤组(74.45±1.62)%、(73.56±1.69)%、(72.54±2.93)%、(3676.32±259.65)U/L,差异均有统计学意义(t=2.357、4.541、6.368、6.388,P值均小于0.05)。丁酸钠组伤后6 h肝和小肠黏膜血流量[(65.36±11.79)、(62.65±12.56)BPU]分别高于烧伤组[(51.72±10.54)、(31.56±12.72)BPU],差异均有统计学意义(t=2.439、4.919,P值均小于0.05);肺、肝、肾和小肠含水率[(73.72±2.05)%、(78.41±1.84)%、(75.64±2.63)%、(70.53±3.13)%]分别低于烧伤组[(80.62±2.16)%、(82.62±1.93)%、(80.32±3.05)%、(81.53±2.79)%],差异均有统计学意义(t=6.553、4.465、3.286、7.420,P值均小于0.05);丁酸钠组血浆ALT(96.36±6.56)U/L、Cr(79.35±4.16)μmol/L、DAO(78.54±5.23)U/L、CK-MB(3712.64±309.45)U/L分别低于烧伤组[(113.54±7.41)U/L、(90.34±5.37)μmol/L、(92.34±5.34)U/L、(5264.46±351.62)U/L],差异均有统计学意义(t=4.910、4.576、5.222、9.370,P值均小于0.05)。结论丁酸钠能够增加特�Objective To investigate the effects of sodium butyrate acid on organ function and visceral blood flow in rats with extraordinarily severe burn injury. Methods Forty-eight male SD rats whose average weight were （ 250 ± 20 ） grams were randomly divided into sham group, burn injury group and sodium butyrate group （n = 16 ）. Rats of burn injury group and sodium butyrate group were subjected to extraordinarily severe burn injury by scalding the rats＇ back 15 s and abdomen 8 s with boiling water. And then sodium butyrate solution （400 mg/kg） or same volume of 0. 9% sodium chloride solution was intraperitoneally injected, respectively. While the same volume of 0. 9% sodium chloride solution was intraperitoneally injected into rats of sham group after soaking in 37 ℃ warm water. At 3 and 6 hours after injury, the blood flow of liver, kidney and small intestine were measured by laser doppler flowmetry, and blood samples were drawn from abdominal aorta for measurement of parameters of organ function and the activity of diamine oxidase. The tissues samples of kidney, liver, heart, lung and small intestine were harvested for the measurement of tissue water content. Results Compared with burn injury group at 3 hours after injury, the blood flow of kidney and small intestine in sodium butyrate group were （93. 56 ± 11.56） BPU and （ 84.65 ± 12.64 ） BPU, significantly higher than those in burn injury group [ （ 80.71 ± 10.53 ） BPU, （59.64 ± 11.82） BPU ], the difference was statistically significant （ t = 2. 324, 4. 087, all P values were less than 0.05 ）. The water content of heart, lung and small intestine in sodium butyrate group were （72.35 ± 1.93 ） %, （69.56 ± 1.83 ） %, （63.75 ± 2.58 ） %, and the level of creatine kinase MB isoenzyme was （2794.56 ± 291.54 ） U/L, significantly lower than those in burn injury group [ （74.45 ± 1.62） %, （73.56 ± 1.69） %, （72.54 ± 2.93 ） % ] and （ 3676.32 ± 259.65 ） U/L, the difference was statistically significant（

关 键 词：组蛋白脱乙酰基酶抑制剂 烧伤 水肿 丁酸钠 脏器功能 血流量 

分 类 号：R644[医药卫生—外科学]