PRDM16基因启动子区甲基化与超重肥胖发生风险的关联研究  

The exploration of the correlation between the risk of obesity and the promoter methylation of PRDM16 gene

作　　者：孙盼盼[1] 刘莉[2] 詹芳芳[3] 戚敏杰[1] 卢明[4] 陈远思[5] 陈嘉欣[5] 付晓丽[1] 平智广[1] 

机构地区：[1]郑州大学公共卫生学院流行病与卫生统计学教研室,450001 [2]郑州大学基础医学院组织学与胚胎学系,450001 [3]天津市健康教育所,300011 [4]焦作市人民医院护理部,454150 [5]郑州大学基础医学院,450001

出　　处：《中华内分泌代谢杂志》2016年第5期370-375,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金（81001280,81202277,81373096）

摘　　要：目的：探讨PRDM16（positive regulatory domain containing 16）基因启动子区CpG位点的甲基化水平与肥胖的相关性。方法选取河南省某医院腹部手术患者116例,分为正常体重组50例、超重肥胖组66例,其中成年男性25例、成年女性91例。应用外周血测定空腹血糖、总胆固醇、三酰甘油、高密度脂蛋白和低密度脂蛋白水平；然后提取DNA和亚硫酸氢盐修饰并使用质谱法对PRDM16基因启动子区CpG位点甲基化水平进行检测。最后应用IBM SPSS Statistics 21.0软件进行统计学分析：研究对象基本特征和生化指标资料的组间比较采用两独立样本t检验；性别资料采用χ^2检验；多个CpG位点甲基化与体重指数（BMI）间关系采用多重线性回归；检验水准α=0.05。结果 PRDM16基因启动子区全部有效检测位点CpG5.6、CpG8、CpG9、CpG12、CpG13.14.15、CpG26.27、CpG28和CpG29甲基化水平在正常体重组和超重肥胖组之间无区别（P〉0.05）；经分层分析,超重肥胖组CpG26.27位点与BMI有线性关系,且甲基化程度越高,BMI越大,标准化偏回归系数为46.928（P=0.015）。结论 PRDM16基因启动子区CpG26.27位点甲基化水平可能与肥胖相关。Objective To explore the association between the CpG methylation level of positive regulatory domain containing 16（PRDM16）gene promoter and obesity or body mass index（BMI）. Methods A total of 116 patients（91 female adults and 25 male adults） with abdominal operation in a municipal hospital of Henan province were enrolled in this study and they were divided into two groups：normal weight group（n=50）, overweight or obesity group （ n=66 ） . Fasting plasma glucose, total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein were measured in peripheral blood. DNA was extracted from white blood cells in peripheral blood and modified by bisulphite. Then the CpG methylation level of PRDM16 gene promoter was detected by mass spectrometry. Finally, all data were analyzed by IBM SPSS Statistics 21. 0 at the 5% level. The essential features and biochemical indexes of research objects between two groups were compared by two independent sample t-test, except chi-square test for gender. The correlation between CpG methylation level of PRDM16 gene and BMI was analyzed by multiple linear regression. Results There were no significant differences （ P〉0. 05 ） in the methylation levels of PRDM16 gene＇s effective CpG sites（including CpG5. 6, CpG8, CpG9, CpG12, CpG13. 14. 15, CpG26. 27, CpG28 and CpG29） between two groups. The methylation level of CpG26. 27 had positive linear relation with BMI in overweight or obesity group with the standardized coefficients of 46. 928（P=0. 015）, which means the higher the methylation level is, the higher the BMI would be. Conclusion The CpG26. 27 methylation level of PRDM16 gene promoter region may have relationship with the risk of obesity.

关 键 词：肥胖症 PRDM16 甲基化 

分 类 号：R589.2[医药卫生—内分泌]