小檗碱配伍水苏糖对糖尿病小鼠糖脂代谢及肠道菌群的影响  

Effects of berberine compatible with stachyose on glucolipid metabolism and gut microbiota in diabetic mice

作　　者：韩雨薇[1] 李彩娜[1] 环奕[1] 孙素娟[1] 穆永钊 申竹芳[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050

出　　处：《中国临床药理学杂志》2016年第12期1121-1124,共4页The Chinese Journal of Clinical Pharmacology

基　　金：重大新药创制国家科技重大专项基金资助项目(2012ZX09301002-004)

摘　　要：目的考察小檗碱配伍水苏糖对自发性2型糖尿病KKAy小鼠糖脂代谢及肠道菌群的影响。方法将2型糖尿病KKAy雌性小鼠随机分为模型组(水0.1 m L·10 g^(-1))、小檗碱组(小檗碱100 mg·kg^(-1)·d^(-1))、水苏糖组(水苏糖200 mg·kg^(-1)·d^(-1))及联合组(小檗碱100 mg·kg^(-1)·d^(-1)+水苏糖200mg·kg^(-1)·d^(-1)),每组9只;另随机选取10只C57BL/6J雌性小鼠作为正常对照组(水0.1 m L·10 g^(-1))。5组小鼠每日灌胃给药一次,连续7周。检测小鼠血糖、血脂、口服葡萄糖耐量及胰岛素敏感性的变化,并用基于16SrRNA的实时荧光定量聚合酶链式反应技术分析小鼠肠道中乳酸杆菌及双歧杆菌丰度的变化。结果与模型组相比,联合组可显著降低KKAy小鼠的空腹血糖、血清总胆固醇水平(P<0.05),改善其口服葡萄糖耐量异常(P<0.05)及胰岛素抵抗(P<0.01),还可显著上调小鼠肠道益生菌乳酸杆菌和双歧杆菌丰度(P<0.01),且其作用明显优于小檗碱组及水苏糖组。结论小檗碱配伍水苏糖能明显改善糖尿病KKAy小鼠的糖脂代谢紊乱,且作用效果相比于单一成分更具优越性,其机制可能与促进肠道益生菌乳酸杆菌及双歧杆菌的增殖有关。Objective To evaluate the effects of berberiue compatible with stachyose on glucolipid metabolism and gut microbiota in type 2 diabetic KKAy mice. Methods Female diabetic KKAy mice selected by insulin tolerance test （ITT） were divided into control group （water 0. 1 mL. 10 g-1）, berberine group （berberine 100 mg . kg-1 . d-1）, stachyose group （stachyose 200 mg . kg-1 . d-1） and combined group （berberine 100mg.kg-1. d-1 ＋ stachyose200 mg. kg-1. d-1）, 9 mice per group. Ten female C57BL/6J mice were served as normal control group （water 0. 1 mL . 10 g-1）. All mices were orally administrated once daily for 7 weeks. Fasting plasma glucose and lipids levels were detected. Oral glucose tolerance test （OGqq＇） and ITT were determined at the end of treatment. In addition, the quantities of Lactobacillus and Bifidobacterium in miees intestine were examined by 16SrRNA -targeted real - time fluorescence quantitative polymerase chain reaction. Results Compared with control group, combined group was markedly improved glucose tolerance and insulin sensitivity （ P 〈 0. 01 ）, decreased fasting plasma glucose and total cholesterol levels （P 〈 0. 05 ） and enriched the abundance of Lactobacillus （P 〈 0. 01 ） and Bifidobacterium （ P 〈 0. 01 ）, which were significantly better than berberine group and stachyose group. Conclusion Berberine combined with stachyose provided a better control for glucolipid metabolism than any single component in diabetic KKAy mice, which was related to up - regulation of Lactobacillus and Bifidobacterium in mice intestine.

关 键 词：小檗碱 水苏糖 糖脂代谢 肠道菌群 

分 类 号：R28[医药卫生—中药学]