过表达veA对橘青霉美伐他汀生物合成的影响  

On Investigation of veA Gene in Regulating Mevastatin Biosynthesis,Conidia Development in Penicillium citrinum

作　　者：曹双[1,2] 郑跃亮[1,2] 张虎[1,2] 黄宇琪[1,2] 廖国建[1,2] 胡昌华[1,2] 

机构地区：[1]西南大学药学院,重庆400716 [2]重庆药物过程与质量控制工程技术中心,重庆400716

出　　处：《西南师范大学学报（自然科学版）》2016年第6期67-73,共7页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：重庆科技攻关示范项目(cstc2013jcsf10016)

摘　　要：在丝状真菌中,全局性调控因子VeA广泛存在并高度保守,调节多个次级代谢基因簇和生长发育.在橘青霉中克隆veA的基础上,通过农杆菌介导的转化获得了OE∷veA菌株.结果显示:veA过表达后,分生孢子产量下降61%,美伐他汀产量提高3倍.光照能促进橘青霉中veA的表达从而抑制橘青霉生长发育.这些结果提示在橘青霉中veA和孢子形成、美伐他汀合成密切相关.The global regulatory gene veA,which is conserved in filamentous fungi,is connected with conidia development and secondary metabolism.In this study,aveA-overexpression strain has been constructed via the agrobacterium-mediated transformation of Penicilliumcitrinum,and it has been found that the overexpression of VeA lead to a 61% decreased in conidiaproduction in P.citrinum.Furthermore,the results reveal that VeA positively regulated mevastatin biosynthesis in P.citrinum.In addition,it has been found that light could promote the regulatory effects of veAin conidia development by activating veA expression.Overall this work indicates that the veAortholog in P.citrinumis involved in the control of conidia development,mevastatin biosynthesis.

关 键 词：veA 调控 美伐他汀产量 橘青霉 

分 类 号：Q939.9[生物学—微生物学]