响应面法优化藏药毛莲蒿总香豆素的超声辅助提取工艺  

On Optimization of Ultrasonic Extraction Conditions of Total Coumarins from Tibetan Medicine Artemisia Vestita Wall.by Response Surface Methodology

作　　者：杨正明[1] 滕云[1] 韩亚涛[1] 李波[1] 王景富[1] 刘圆[2] 

机构地区：[1]西南民族大学化学与环境保护工程学院,成都610041 [2]西南民族大学民族医药研究院,成都610041

出　　处：《西南师范大学学报（自然科学版）》2016年第6期74-80,共7页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(81173653);四川省应用基础计划项目(2014JY0181);西南民族大学研究生学位点建设项目(2015XWD-S0703)

摘　　要：通过单因素实验法考察乙醇体积分数、浸提时间、浸提温度、料液比及超声时间对毛莲蒿总香豆素得率的影响,并通过Box-Behnken试验设计优化毛莲蒿总香豆素的超声辅助提取工艺.结果表明,毛莲蒿总香豆素的最佳提取工艺为乙醇体积分数72%,浸提时间71min,浸提温度68℃,料液比1∶58(g/mL)及超声时间30min,在该条件下毛莲蒿总香豆素的平均得率为3.45%.优化后的提取工艺方便、可行,可为毛莲蒿中总香豆素类化合物的提取提供参考.The effects of ethanol concentration,extraction time,extraction temperature,ratio of solid to liquid and ultrasonic time in extraction procedure on the extraction rate of total coumarins in Artemisia vestita Wall have beenresearched by single factor experiment,and the Box-Behnken experimental design has beenused to optimize the extraction of total coumarins in A.vestita Wall.Results show that the optimum extraction conditions are as follows：72% ethanol,extraction time 71 min,extraction temperature 68 ℃,ratio of solid to liquid 1∶58（g/mL）and ultrasonic time 30 min.Under the conditions,the average extraction yield of total coumarins reaches 3.45%.The optimized method is convenient and feasible,which can provide reference for the extraction of total coumarins fromA.vestita Wall.

关 键 词：毛莲蒿 总香豆素 响应面法 超声波辅助 提取工艺 

分 类 号：R931[医药卫生—生药学]