Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean  

作　　者：Yuan Qiang Wei Yun-min Fu Ming-ming Qiu You-wen Wen Hong-tao Zhang Ming-hui Liu Ying Ao Jin-xia 

机构地区：[1]College of Life Sciences, Northeast Agricultural University, Harbin 150030, China [2]Heilongjiang Forest Protection Research Institute, Harbin 150040, China [3]Heilongjiang Institute of Education, Harbin 150080, China [4]Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2016年第2期45-51,共7页东北农业大学学报（英文版）

摘　　要：A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows： the concentration of digoxin tag probe was 0.5 μmol · L^-1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows： the concentration of digoxin tag probe was 0.5 μmol · L^-1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.

关 键 词：PCR ELISA glyphosate resistant transgenic soybean 

分 类 号：S565.1[农业科学—作物学] S451