利用秀丽隐杆线虫建立灵芝生物学功效的评价方法  

Establishment of Quality Evaluation Indicator System for Ganoderma lucidum by Enhancing the Antioxidant Capacity of Caenorhabditis elegans

作　　者：VU THI CUONG 陈伟东[1] 杨平 费俭[1,2] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]上海南方模式生物研究中心,上海201203

出　　处：《中国细胞生物学学报》2016年第5期499-509,共11页Chinese Journal of Cell Biology

基　　金：上海市科委项目(批准号:13DZ2280600)资助的课题~~

摘　　要：中药的质量评价是中药应用的关键。目前,大部分中药的活性物质尚不清楚且其成分较为复杂,使得以化学检测为手段的中药质量标准的建立存在很大的盲目性。建立生物学功效的检测方法,可以为中药的质量评价提供一条新的路径。该文利用秀丽隐杆线虫(Caenorhabditis elegans)评价了五种不同产地灵芝水提取物对提高野生型线虫和FOXO(forkhead box O)转录因子突变体抗氧化应激能力的效果。结果表明,不同产地灵芝均能够提高线虫抗氧化应激的能力,但功效差异显著。同时,不同产地灵芝提高线虫氧化应激能力的机制也存在差异,这提示了灵芝中存在不同的起效活性物质。该文的研究结果证明了利用模式生物秀丽隐杆线虫可以有效地区分不同产地灵芝的生物活性,并可以将其作为探索评价灵芝生物活性的一个研究方向。Quality evaluation of TCM （traditional Chinese medicine） is the key to its application. Currently, the chemical method such as Mass Spectrometry is the main method to quantify the traditional Chinese medicine. But the TCM has a complicated composition, and the functional components of most TCM remains unclear, which makes it sightless to establish the quality standard of traditional Chinese medicine by chemical methods. So, using living organism may provide a new approach to evaluate TCM＇s quality. In this work, we treat both wild type and FOXO （forkhead box O） transcription factor mutant Caenorhabditis elegans with water extracts of five Ganodorma lucidum from different area. The results show that all of the extracts can enhance the antioxidant capacity, but the work concentration of these five extracts differs greatly from each other. Moreover, after treating with the extracts from different area Or the same extract of different concentration, the mechanism is different, indicating that the water extracts of Ganoderma lucidum have various bioactive components. Our work proved that we could use Caenorhabditis elegans to efficiently detect the biological activity of Ganoderma lucidum, and it also provided a new method to evaluate the bioactivity of Ganoderma lucidum from different areas.

关 键 词：灵芝 中药 质量评价 氧化应激 秀丽隐杆线虫 

分 类 号：R285.5[医药卫生—中药学]