G蛋白γ13亚单位在发育的嗅上皮和梨鼻中的表达模式研究（英文）  

The G Protein γ-subunit Gγ13 Is Expressed in the Developing Olfactory and Vomeronasal Neurons

作　　者：王小云[1] 梁洁[1] 顾复[1] 陈佳莹[1] 陈付学[1] 李峰[2] 

机构地区：[1]上海大学生命学院,上海200469 [2]上海市(复旦大学附属)公共卫生临床中心,上海201508

出　　处：《中国细胞生物学学报》2016年第5期527-539,共13页Chinese Journal of Cell Biology

基　　金：上海市科委项目(批准号:15DZ2290200)资助的课题~~

摘　　要：G蛋白亚单位以前被认为在味蕾中特异性的表达,和味导素、苦味受体共表达于味蕾的II型细胞。目前的研究发现,Gγ13(G proteinγ-subunit Gγ13)在小鼠不同发育时期嗅上皮和梨鼻均存在表达,包括胚胎期15.5 d(E15.5)、生后期第0 d(P0)、生后期第5 d(P5)、生后期第10 d(P10)、生后期第21 d(P21)和成年期(>P40)。研究也表明,Gγ13可能是一个成熟嗅神经和梨鼻神经的分子标记物。m RNA原位杂交表明,Gγ13和Gα亚单位Gαolf(Gαolf在成熟嗅神经细胞中表达)的表达模式在嗅上皮是一致的,Gγ13和Gα亚单位Gαi2(Gαi2在成熟梨鼻嗅神经细胞中表达)在梨鼻上皮共定位。Gγ13的分布不同于标记细胞发育的标记物GAP43(growth associated protein 43)在嗅上皮的分布,它的表达也不同于另外一个G蛋白亚单位Gγ8的表达分布。在P21的嗅觉系统,Gγ13蛋白在嗅上皮嗅毛中表达丰富,在梨鼻的嗅毛表达也丰富。在主嗅球,在颗粒细胞带、外网层、僧帽细胞带均发现Gγ13的阳性信号。而且,m RNA原位杂交也显示,Gγ13在僧帽细胞带表达,表明Gγ13可能参与到僧帽细胞向大脑嗅皮质区的信号输送。在副嗅球,在颗粒细胞层发现微弱的阳性信号。总之,目前的研究表明,Gγ13可能参与嗅上皮和梨鼻的嗅分子信号传导过程。The tissue localization of the G protein y-subunit Gγ13 that has been believed to be specifically expressed in taste bud before, was studied in the olfactory and vomeronasal neurons at different ages： embryonic day 15.5, postnatal days 0, 5, 10, 21 and adult. Gγ13 appears to be a specific marker of the mature olfactory and vomeronasal neurons. In situ hybridization （ISH） reveals that Gγ13 distribution is identical to that of Gαolf, which is predominantly expressed in main olfactory epithelia （MOE）, and that of Gαi2, which is predominantly expressed in mature vomeronasal organ （VNO）. Gγ13 distribution differs from that of growth associated protein 43 （GAP43）, and that of Gγ8. Gγ13 proteins are enriched in cilia of MOE and mierovilli of VNO after P21 days. In main olfactory bulb （MOB）, Gγ13-positive signals were present in the glomerular layer （GL）, external plexiform layer （EPL） and mitral cell layer （MCL）. Furthermore, the expression of Gγ13 was also detected in mitral cell layer with mRNA ISH, indicating that Gγ13 may be involved in the output of mitral cells to various parts of the olfactory cortex. In accessory olfactory bulb （AOB）, the weak positive signals were also observed in the glomerular layer. In short, the current results collectively suggest that Gγ13 appear to be involved in signal transduction of MOE and VNO.

关 键 词：G蛋白 嗅神经 梨鼻 主嗅球 副嗅球 小鼠 

分 类 号：Q344[生物学—遗传学]