JOINTLESS/MACROCALYX/SLMBP21多基因转化番茄植株表型鉴定与分析  

Characterizing the JOINTLESS/MACROCALYX/SLMBP21 Multi-gene Overexpression Transgenic Plant of Tomato

作　　者：刘旦梅[1] 张彦洁[1] 裴雁曦[1] 

机构地区：[1]山西大学生命科学学院,太原030006

出　　处：《中国细胞生物学学报》2016年第5期557-565,共9页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31501772)资助的课题~~

摘　　要：器官脱落是植物中重要而普遍的生理现象,同时又是一个重要的农艺性状。离区是器官脱落发生的部位,离区发育是研究器官脱落的重要环节。植物MADS-box蛋白是一类在植物生长发育中起关键作用的转录因子,参与多种生理过程。已有报道表明,番茄花(果)柄离区发育受MADS-box蛋白JOINTLESS(J)、MACROCYLYX(MC)和SLMBP21的调控,且这3个蛋白之间可发生两两互作,但由这3个蛋白所形成的蛋白复合体是否足以决定番茄花(果)柄离区形成尚且未知。在该研究中,作者利用一个多基因共表达植物转化系统在番茄植株中同时过表达J、MC和SLMBP21这3个基因,并成功获得了J/MC/SLMBP21多基因过表达(3GOX)转基因植株。通过转基因植株表型观察发现,转基因植株的果梗和果柄发生同源异型改变,转变为类似于离区的结构,但植株其他部位没有明显表型,说明J-MC-SLMBP21蛋白复合体在果梗和果柄中可以诱导离区的形成,但也暗示果梗和果柄中可能存在其他与J、MC和SLMBP21处于同一作用层面的因子。同时,通过在3GOX植株中检测J、MC和SLMBP21的下游基因表达水平,找到了4个可能与离区细胞形成有关的基因(Le WUS、Bl、GDSL和HMG1)。这些研究结果为离区发育分子机制的深入研究提供了线索,奠定了基础。Abscission is a ubiquitous and dynamic process during the life cycle of a plant, and it is also a critical agricultural trait. Abscission zone （AZ） is the place for abscission, and AZ formation is an important part for the study of abscission. Plant MADS-box proteins are a kind of crucial transcription factors involved in various developmental and physiological processes. Previously, it was reported that the development of the flower （fruit） AZ of tomato was controlled by JOINTLESS（J）, MACROCYLYX（MC） and SLMBP21, three MADS-box proteins which could also interact with each other. However, whether the J-MC-SLMBP21 protein complex is enough to induce the formation of AZ is still under investigation. Here, we successfully got the J/MC/SLMBP21 multigene overexpression plant （3GOX） through using a multigene assembly and transformation vector system. And through phenotype analysis, we found that the fruit peduncle and pedicel of the transgenic plant were all converted into AZlike structures, indicating that the J-MC-SLMBP21 protein complex was able to induce the formation of AZ in the peduncle and pedicel. But the result also hinted the existence of other factors functioning in the same level with J, MC and SLMBP21 in these two tissues. Moreover, the expression level detection of the downstream genes demon- strated that LeWUS, BI, GDSL and HMG1 might be involved in the formation of AZ cells. Our study provided the clues and made a foundation for further study of the molecular mechanisms under AZ development.

关 键 词：JOINTLESS MACROCYLYX SLMBP21 多基因共表达 离区发育 

分 类 号：Q943.2[生物学—植物学]