EDTA修复肉芽肿组织中荧光定量PCR检测结核杆菌的应用  

Application of fluorescence quantitative PCR detection of mycobacterium tuberculosis retrieved with EDTA in granuloma tissue

作　　者：王旭洲[1] 谢飞来[1] 许文静[1] 郑巧玲[1] 齐兴峰[1] 郑智勇[1] 

机构地区：[1]南京军区福州总医院病理科,福州350025

出　　处：《临床与实验病理学杂志》2016年第6期665-668,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的观察EDTA热修复炎性肉芽肿组织石蜡切片,能否提高荧光定量PCR结核/非结核分枝杆菌的检出率。方法对125例炎性肉芽肿组织石蜡切片结核/非结核分枝杆菌同时进行常规荧光定量PCR检测和EDTA热修复后荧光定量PCR检测。结果常规荧光定量PCR检测检出分枝杆菌75例(60%)阳性,其中结核杆菌74例(59.2%),非结核分枝杆菌1例(0.8%),阳性病例平均阳性基因拷贝数6.35×105/ml/例;EDTA修复后荧光定量PCR检测检出分枝杆菌88例(70.4%),其中结核杆菌83例(66.4%),非结核分枝杆菌5例(4%),阳性病例平均阳性基因拷贝数7.36×106/ml/例。两组间差异均有统计学意义(P<0.05,P<0.01)。结论 EDTA热修复后荧光定量PCR检测可以大幅度提高炎性肉芽肿组织石蜡切片的结核/非结核分枝杆菌的阳性检出率。Purpose To observe the effects of EDTA heat retrieval on the paraffin section of inflammatory granuloma tissue, to determine if it can improve the detective rate of tuberculosis/non-tuberculosis mycobacterium by fluorescent quantitative PCR. Methods Tuberculosis/non-tuberculosis mycobacteria were also examined by conventional fluorescence quantitative PCR and fluorescent quantitative PCR detection with EDTA heat retrieval in 125 cases of inflammatory granuloma. Results Conventional fluorescence quantitative PCR detection of mycobacterium was positive in 75 cases （60%）, including mycobacterium tuberculosis （74 cases, 59.2% ）, and non-tuberculous mycobacteria （ 1 case, 0. 8% ） ; the average positive gene copy numbers were 6. 35 × 10^5/ml/cases. EDTA retrieval fluorescence quantitative PCR detection of mycobacteria were positive in 88 cases （70. 4% ）, including 83 cases of Mycobacterium tuberculosis （66.4%）, 5 cases of non-tuberculous mycobacteria （4%）, and the average positive gene copy number was 7. 36 × 10^6/ ml/cases. The difference between the two groups was statistically significant （ P 〈 0. 05, P 〈 0.01 ）. Conclusion EDTA heat-retrieved fluorescence quantitative PCR detection can greatly improve the positive rates of tuberculosis/non-tuberculosis mycobacterium in paraffin-embedded tissues.

关 键 词：结核肉芽肿组织 EDTA修复 荧光定量PCR检测 结核/非结核分枝杆菌 

分 类 号：R52[医药卫生—内科学] R446[医药卫生—临床医学]