人卵母细胞辅助激活研究进展  

Current achievments of assisted oocyte activation in human

作　　者：贾洪响[1] 苏丹[1] 毕星宇[1] 武学清[1] 

机构地区：[1]山西省妇幼保健院生殖医学中心,太原030000

出　　处：《生殖与避孕》2016年第6期500-505,共6页Reproduction and Contraception

摘　　要：卵胞质内精子注射（intracytoplasmicsperminjection，ICSI）是目前治疗男性不育最有效的方法．但在ICSI周期中仍然有1％～3％的受精失败发生。近年来逐渐发展起来的卵母细胞辅助激活（assistedoocyteactivation，AOA）技术在改善ICSI后受精失败患者的妊娠结局方面取得了较为明显的效果，有望成为治疗男性不育的重要辅助手段。本文综述了卵母细胞激活机制领域的研究成果，揭示了精子中的PLC三蛋白引起卵母细胞内Ca2＋震荡进而触发激活的机制，介绍了目前对ICSI受精失败患者精子激活能力的检测与诊断方法，以及不同卵母细胞辅助激活技术如电激活，Ca2＋载体和SrClz等激活技术的研究现状，并总结了这些技术在安全性方面所获得的证据，在此基础上对AOA技术今后的发展方向和前景作了探讨。Even though intracytoplasmic sperm injection （ICSI） is thought to be the most effective treatment for male infertility, 1%-3% fertilization failure still occured following ICSI. Assisted oocyte activation （AOA） has been developed for improving pregnancy outcome of patients suffering fertilization failure following ICSI, and been possible to become an important assisted technology in male infertility treatment. We reviewed the achievements in oocyte activation mechanism of human, and described the mechanism that PLC~ in sperm activated oocyte by causing intracellular Ca2~ oscillations, and introduced the diagnostic tools for sperm activating capability of patients, and the current research status of different AOA technologies, such as electrical activation, Ca2＋ ionophore, and SrC12 activation, and furthermore we summarized evidences in safety of AOA technologies, and then discussed prospect and development of AOA technology in future.

关 键 词：辅助激活(AOA) Ca2+震荡 受精失败 人卵母细胞 

分 类 号：R698.2[医药卫生—泌尿科学]