RP-HPLC法同时测定蒙药蓝盆花中木犀草素和芹菜素的含量  

Simultaneous Determination of Luteolin and Apigenin in Mongolian Medicine Scabiosa atropurea by RP-HPLC

作　　者：王美丽[1] 田香[1] 白玉霞[1] 

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028300

出　　处：《中国药房》2016年第18期2540-2542,共3页China Pharmacy

基　　金：国家科技支撑计划项目(No.2012BAI28B01);内蒙古自治区科技计划项目(No.2010NMKJJH15)

摘　　要：目的：建立同时测定蒙药蓝盆花中木犀草素和芹菜素含量的方法。方法：采用反相高效液相色谱法。色谱柱为Diamond C18，流动相为乙腈-0.4％磷酸（34∶66，V/V），流速为1.0 ml/min，检测波长为350 nm，柱温为30℃，进样量为10μl。结果：木犀草素和芹菜素的检测进样量线性范围分别为66～396 ng（r＝0.9998）、93～558 ng（r＝0.9996）；精密度、稳定性、重复性试验的RSD＜2％；加样回收率分别为98.15％～101.79％（RSD＝1.42％，n＝6）、98.66％～104.05％（RSD＝1.81％，n＝6）。结论：该方法操作简便，精密度、稳定性、重复性好，可用于同时测定蒙药蓝盆花中木犀草素和芹菜素的含量。OBJECTIVE：To establish a method for simultaneous determination of luteolin and apigenin in Mongolian medicine Scabiosa atropurea. METHODS：HPLC was performed on the column of Diamond C18 with mobile phase of acetonitrile-0.4%phos-phoric acid（34∶66,V/V）at a flow rate of 1.0 ml/min,the detection wavelength was 350 nm,column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS：The linear range was 66-396 ng for luteolin（r=0.999 8）and 93-558 ng for apigenin （r=0.999 6）;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.15%-101.79%（RSD=1.42%,n=6） and 98.66%-104.05%（RSD=1.81%,n=6）,respectively. CONCLUSIONS：The method is simple with good precise,stability and reproducibility,and can be used for the simultaneous determination of luteolin and apigenin in Mongo-lian medicine S. atropurea.

关 键 词：蒙药蓝盆花 木犀草素 芹菜素 反相高效液相色谱法 

分 类 号：R927[医药卫生—药学]