HPLC法测定白芥子饮片中白芥子苷的含量  

Content Determination of Sinalbin in Sinapis alba Decoction Piece by HPLC

作　　者：徐敏[1] 叶凡[2] 赖先荣[2] 杨艳[1] 

机构地区：[1]成都市第三人民医院中药剂科/重庆医科大学附属成都第二临床学院,成都610031 [2]成都中医药大学民族医药学院,成都611137

出　　处：《中国药房》2016年第18期2549-2551,共3页China Pharmacy

基　　金：成都市卫生局科研课题(No.2013051)

摘　　要：目的：建立测定白芥子饮片中白芥子苷含量的方法。方法：采用高效液相色谱法。色谱柱为Sepax HP-C18，流动相为50％乙腈-水溶液（含5 mmol/L四正辛基溴化铵），流速为1.0 ml/min，检测波长为227 nm，柱温为30℃，进样量为10μl。结果：白芥子苷的检测质量浓度线性范围为0.0101～0.2024 mg/ml（r＝0.9997）；精密度、稳定性、重复性试验的RSD＜2％；加样回收率为98.51％～104.62％，RSD＝1.90％（n＝9）。结论：该方法操作简便、结果准确可靠，可用于白芥子饮片中白芥子苷的含量测定。OBJECTIVE：To establish a method for the content determination of sinalbin in Sinapis alba decoction piece. METHODS：HPLC was performed on the column of Sepax HP-C18 with mobile phase of 50% acetonitrile-water solution（contain-ing 5 mmol/L tetraoctyl ammonium bromide）at a flow rate of 1.0 ml/min,detection wavelength was 227 nm,column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS：The linear range of sinalbin was 0.010 1-0.202 4 mg/ml（r=0.999 7）;RSDs of precision,stability and reproducibility tests were lower than 2%,recovery was 98.51%-104.62%（RSD=1.90%,n=9）. CONCLUSIONS：The method is simple,accurate and reliable,and can be used for determination of sinalbin in Sinapis alba decoc-tion piece.

关 键 词：白芥子 白芥子苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]