HPLC法测定山柰药材中山柰素的含量  

Content Determination of Kaempferol in Kaempferia galanga by HPLC

作　　者：李刚[1] 封传华[1] 张静[1] 徐兰[1] 陶晓璇[1] 

机构地区：[1]解放军第94医院,南昌330002

出　　处：《中国药房》2016年第18期2558-2559,共2页China Pharmacy

摘　　要：目的：建立测定山柰药材中山柰素含量的方法。方法：采用高效液相色谱法。色谱柱为Diamonsil ODS2 C18，流动相为甲醇-0.4％磷酸溶液，流速为1 ml/min，检测波长为367 nm，柱温为30℃，进样量为10μl。结果：山柰素的检测质量浓度线性范围为0.00158～0.158 mg/ml；精密度、稳定性、重复性试验的RSD＜3％；加样回收率为95.52％～99.32％，RSD＝1.47％（n＝6）。结论：该方法操作简单、结果准确、重复性较好，可用于山柰药材中山柰素的含量测定。OBJECTIVE：To establish a method for the content determination of kaempferol in Kaempferia galanga. METH-ODS：HPLC was performed on the column of Diamonsil ODS2 C18 with mobile phase of methanol-0.4% Phosphoric acid solution at a flow rate of 1 ml/min,detection wavelength was 367 nm,column temperature was 30℃,and injection volume was 10 μl. RE-SULTS：The linear range of kaempferol was 0.001 58-0.158 mg/ml;RSDs of precision,stability and reproducibility tests were low-er than 3%;recovery was 95.52%-99.32%（RSD=1.47%,n=6）. CONCLUSIONS：The method is simple,accurate and reproduc-ible,and can be used for the content determination of kaempferol in K. galanga.

关 键 词：高效液相色谱法 山柰 山柰素 

分 类 号：R917[医药卫生—药物分析学]